{"id":"3c846bb9-42c7-5ddb-b209-55aee1a45801","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-gpr50-mt1-heteromer","predicate":"heterodimerizes_with","statement":"GPR50 formed heterodimers with MT1 in the tested cell systems.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d01446f9-6399-5f26-8466-c57f96953873","mechanism_event_label":"A receptor partner can alter access to a signaling route.","subject":{"id":"f006a09d-bba6-5ea0-88d7-79eaddd59cf3","slug":"gpr50","display_name":"Human melatonin-related orphan receptor / GPR50","entity_type_key":"protein"},"object":{"id":"dff2050f-ecec-53a7-8e40-8cc2ad2da882","slug":"mtnr1a","display_name":"Human melatonin receptor 1A / MT1 / MTNR1A","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d01446f9-6399-5f26-8466-c57f96953873","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-gpr50-mt1-heteromer-event","event_type":"biochemical_relationship","label":"A receptor partner can alter access to a signaling route.","description":"GPR50 formed heterodimers with MT1 in the tested cell systems.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f006a09d-bba6-5ea0-88d7-79eaddd59cf3","slug":"gpr50","display_name":"Human melatonin-related orphan receptor / GPR50","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dff2050f-ecec-53a7-8e40-8cc2ad2da882","slug":"mtnr1a","display_name":"Human melatonin receptor 1A / MT1 / MTNR1A","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/melatonin-research/16778767.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"19c9ae903fb7926bb21cc3bda0a610612b16a899f850b8e787581b72ac434135\", \"start_char\": 0, \"end_char\": 1204, \"text_sha256\": \"19c9ae903fb7926bb21cc3bda0a610612b16a899f850b8e787581b72ac434135\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical and biophysical receptor heteromer experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Full-length versus C-terminally truncated GPR50","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-system regulation; does not establish that a person with poor sleep has excess GPR50 or that more melatonin overcomes it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Melatonin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human receptors in cultured cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A receptor partner can alter access to a signaling route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[melatonin-p16778767] The orphan GPR50 receptor specifically inhibits MT1 melatonin receptor function through heterodimerization. (2006). https://pubmed.ncbi.nlm.nih.gov/16778767/ DOI: 10.1038/sj.emboj.7601193","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"GPR50-MT1/MT2 interactions","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ddaa9326-dc13-50e4-94ad-d489e9bec156","evidence_kind":"source_excerpt","locator":"Lines 422-433","start_line":422,"end_line":433,"excerpt":"### melatonin-gpr50-mt1-heteromer\nGPR50 formed heterodimers with MT1 in the tested cell systems.\nCondition category: normal\nnutrient_topic: Melatonin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A receptor partner can alter access to a signaling route.\norganism: Human receptors in cultured cells\ntissue_or_cell_type: GPR50-MT1/MT2 interactions\nexperimental_model: Biochemical and biophysical receptor heteromer experiments\nlimitations: Cell-system regulation; does not establish that a person with poor sleep has excess GPR50 or that more melatonin overcomes it.\nexposure: Full-length versus C-terminally truncated GPR50\nevidence_span: {\"source_cache\": \"artifacts/melatonin-research/16778767.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"19c9ae903fb7926bb21cc3bda0a610612b16a899f850b8e787581b72ac434135\", \"start_char\": 0, \"end_char\": 1204, \"text_sha256\": \"19c9ae903fb7926bb21cc3bda0a610612b16a899f850b8e787581b72ac434135\"}\n[melatonin-p16778767] The orphan GPR50 receptor specifically inhibits MT1 melatonin receptor function through heterodimerization. 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