{"id":"3c6a46d2-3dc1-558c-81b2-d94b82a887b3","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-depletion-intercalated-glul","predicate":"depletion-increases","statement":"The same hypokalemic diet increased glutamine synthetase expression in type A intercalated cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b620c6f2-beee-5061-9391-736c1afbd4ff","mechanism_event_label":"Different kidney cells adjusted the same ammonia-recycling enzyme in opposite directions.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"d38cfc9c-9372-5e5f-8d91-03098155a3c6","slug":"glul","display_name":"Glutamine synthetase / GLUL","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b620c6f2-beee-5061-9391-736c1afbd4ff","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-depletion-intercalated-glul-event","event_type":"biochemical_relationship","label":"Different kidney cells adjusted the same ammonia-recycling enzyme in opposite directions.","description":"The same hypokalemic diet increased glutamine synthetase expression in type A intercalated cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"role":"upstream","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d38cfc9c-9372-5e5f-8d91-03098155a3c6","slug":"glul","display_name":"Glutamine synthetase / GLUL","entity_type_key":"protein"},"role":"downstream","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"recycled-product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e923077b-9786-5ab9-b23b-638777ac6a85","slug":"ammonium-ion","display_name":"Ammonium ion","entity_type_key":"ion"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"endpoint","value_text":"The same hypokalemic diet increased glutamine synthetase expression in type A intercalated cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Mice fed a nominally potassium-free diet for 12 days; renal immunolabeling and protein/RNA assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mice fed a nominally potassium-free diet for 12 days; renal immunolabeling and protein/RNA assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-specific regulation is not a contradiction of the proximal-tubule result; neither establishes net flux in an intact human kidney.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Different kidney cells adjusted the same ammonia-recycling enzyme in opposite directions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[verlander-2013-gs] Expression of glutamine synthetase in the mouse kidney: localization in multiple epithelial cell types and differential regulation by hypokalemia (2013). https://pubmed.ncbi.nlm.nih.gov/23804452/ DOI: 10.1152/ajprenal.00030.2013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"type A intercalated cells of cortical and outer medullary collecting duct","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"79094a5a-9222-5903-8761-147989e51d00","evidence_kind":"source_excerpt","locator":"Lines 1055-1066","start_line":1055,"end_line":1066,"excerpt":"### k-depletion-intercalated-glul\nThe same hypokalemic diet increased glutamine synthetase expression in type A intercalated cells.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Different kidney cells adjusted the same ammonia-recycling enzyme in opposite directions.\norganism: Mus musculus\ntissue_or_cell_type: type A intercalated cells of cortical and outer medullary collecting duct\nexperimental_model: Mice fed a nominally potassium-free diet for 12 days; renal immunolabeling and protein/RNA assays.\nlimitations: Cell-specific regulation is not a contradiction of the proximal-tubule result; neither establishes net flux in an intact human kidney.\nexperimental-exposure: Mice fed a nominally potassium-free diet for 12 days; renal immunolabeling and protein/RNA assays.\nendpoint: The same hypokalemic diet increased glutamine synthetase expression in type A intercalated cells.\n[verlander-2013-gs] Expression of glutamine synthetase in the mouse kidney: localization in multiple epithelial cell types and differential regulation by hypokalemia (2013). https://pubmed.ncbi.nlm.nih.gov/23804452/ DOI: 10.1152/ajprenal.00030.2013","model_system":"Mice fed a nominally potassium-free diet for 12 days; renal immunolabeling and protein/RNA assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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