{"id":"3c68d158-8f10-5b8e-9ea9-a8ead539bb1b","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-fish-target-specificity","predicate":"alters","statement":"Up to 5 micromolar V10 did not inhibit measured F0F1-ATPase activity, NADH levels or complexes I/II, while complex-III redox steady state changed.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"023aeb99-2c63-50ec-9219-8466bd06facb","mechanism_event_label":"Respiratory inhibition did not mean every mitochondrial enzyme was inhibited.","subject":{"id":"ab633bfc-d259-5deb-ac81-a3383f79d4bf","slug":"decavanadate","display_name":"Decavanadate(V)","entity_type_key":"chemical_species"},"object":{"id":"85517e38-5b29-5e20-bd97-3f21894648bd","slug":"sparus-aurata-complex3-redox","display_name":"Complex III redox state in fish cardiac mitochondria","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"023aeb99-2c63-50ec-9219-8466bd06facb","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-fish-target-specificity-event","event_type":"observed_relationship","label":"Respiratory inhibition did not mean every mitochondrial enzyme was inhibited.","description":"Up to 5 micromolar V10 did not inhibit measured F0F1-ATPase activity, NADH levels or complexes I/II, while complex-III redox steady state changed.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab633bfc-d259-5deb-ac81-a3383f79d4bf","slug":"decavanadate","display_name":"Decavanadate(V)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"85517e38-5b29-5e20-bd97-3f21894648bd","slug":"sparus-aurata-complex3-redox","display_name":"Complex III redox state in fish cardiac mitochondria","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"667e5eb4-8764-560f-819b-098e9f7fed2f","slug":"sparus-aurata-mitochondrial-respiration","display_name":"Respiration of isolated Sparus aurata heart mitochondria","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated fish cardiac mitochondrial assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Complex-III redox change does not itself establish a direct molecular binding target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Respiratory inhibition did not mean every mitochondrial enzyme was inhibited.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mitochondria as a target for decavanadate toxicity in Sparus aurata heart. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17420061/ · DOI 10.1016/j.aquatox.2007.03.005","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2f1156de-9daa-578c-bd39-2c7e5a66361d","evidence_kind":"source_excerpt","locator":"Lines 318-324","start_line":318,"end_line":324,"excerpt":"## vanadium-fish-target-specificity\nRespiratory inhibition did not mean every mitochondrial enzyme was inhibited.\nUp to 5 micromolar V10 did not inhibit measured F0F1-ATPase activity, NADH levels or complexes I/II, while complex-III redox steady state changed.\nModel: Isolated fish cardiac mitochondrial assays.\nLimitations: Complex-III redox change does not itself establish a direct molecular binding target.\nEvidence access: Primary abstract\nMitochondria as a target for decavanadate toxicity in Sparus aurata heart. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17420061/ · DOI 10.1016/j.aquatox.2007.03.005","model_system":"Isolated fish cardiac mitochondrial assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"d36b30d972e756ca4d19b66f976980e206c65025bd4bbf4c127c17320bd019c2","revision_id":"70d42967-8032-5627-8670-f59841735e89","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}