{"id":"3c2c8e16-a459-502c-9a52-81a64e82ac57","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-pdac-carbon-tracing","predicate":"supplies_carbon_to","statement":"Carbon-13 cystine tracing showed labeling of glutathione and CoA over the experiment, including CoA labeling over 24 hours.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e5a1e82d-a2e6-5972-8ba0-ae7ea40b733f","mechanism_event_label":"Cysteine-derived material entered more than one protective metabolic pool.","subject":{"id":"cdd84f4d-3e8f-502a-a8c5-19672cc5346f","slug":"cystine","display_name":"Cystine","entity_type_key":"small_molecule"},"object":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"e5a1e82d-a2e6-5972-8ba0-ae7ea40b733f","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-pdac-carbon-tracing-event","event_type":"observed_relationship","label":"Cysteine-derived material entered more than one protective metabolic pool.","description":"Carbon-13 cystine tracing showed labeling of glutathione and CoA over the experiment, including CoA labeling over 24 hours.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cdd84f4d-3e8f-502a-a8c5-19672cc5346f","slug":"cystine","display_name":"Cystine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human PDAC stable-isotope tracing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No labeling of taurine, lactate, citrate or glutamate was detected in these assays; that does not abolish those routes in other tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Cysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Cysteine-derived material entered more than one protective metabolic pool.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cysteine depletion induces pancreatic tumor ferroptosis in mice. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32241947/ · DOI 10.1126/science.aaw9872","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2a535b6a-bbe7-57fa-9ff8-daa9513b2518","evidence_kind":"source_excerpt","locator":"Lines 188-194","start_line":188,"end_line":194,"excerpt":"## l-cysteine-pdac-carbon-tracing\nCysteine-derived material entered more than one protective metabolic pool.\nCarbon-13 cystine tracing showed labeling of glutathione and CoA over the experiment, including CoA labeling over 24 hours.\nModel: Human PDAC stable-isotope tracing.\nLimitations: No labeling of taurine, lactate, citrate or glutamate was detected in these assays; that does not abolish those routes in other tissues.\nEvidence access: Primary full text\nCysteine depletion induces pancreatic tumor ferroptosis in mice. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32241947/ · DOI 10.1126/science.aaw9872","model_system":"Human PDAC stable-isotope tracing.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"654560f6-8d7f-596b-8722-48d94053cfe3","stable_key":"import-a8baf7e9-80e4-5d8c-adec-9a63e84d2f21","title":"L-Cysteine: sulfur allocation, redox supply and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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