{"id":"3c0886cc-a8fe-562e-8ee6-0338c036adab","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-plc-block","predicate":"attenuates","statement":"PLC inhibitor U73122 attenuated CLA-induced calcium accumulation, inflammatory signaling and impaired glucose uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5cd820e5-eca0-5a8b-9da8-5b1ae153d4ed","mechanism_event_label":"A phospholipase-sensitive step linked membrane signaling to calcium.","subject":{"id":"ffe25d58-72d0-52a5-a83e-f7898f5c3ac3","slug":"u73122","display_name":"U73122, pharmacological PLC inhibitor","entity_type_key":"small_molecule"},"object":{"id":"32e78251-e021-5b6f-9366-8be31c98d657","slug":"human-adipocyte-calcium-signal","display_name":"Human adipocyte intracellular calcium response","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5cd820e5-eca0-5a8b-9da8-5b1ae153d4ed","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-plc-block-event","event_type":"observed_relationship","label":"A phospholipase-sensitive step linked membrane signaling to calcium.","description":"PLC inhibitor U73122 attenuated CLA-induced calcium accumulation, inflammatory signaling and impaired glucose uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ffe25d58-72d0-52a5-a83e-f7898f5c3ac3","slug":"u73122","display_name":"U73122, pharmacological PLC inhibitor","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"32e78251-e021-5b6f-9366-8be31c98d657","slug":"human-adipocyte-calcium-signal","display_name":"Human adipocyte intracellular calcium response","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4b863610-cd78-5f40-a114-e4c8b7b7d96e","slug":"plcg1","display_name":"PLCG1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human adipocyte inhibitor experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Broad inhibitor evidence does not isolate PLC-gamma1 as necessary.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A phospholipase-sensitive step linked membrane signaling to calcium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The phospholipase C inhibitor U73122 attenuates trans-10, cis-12 conjugated linoleic acid-mediated inflammatory signaling and insulin resistance in human adipocytes. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23468551/ · DOI 10.3945/jn.112.173161","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eebc74ab-a337-5f16-9ebc-fb2c92fe6e50","evidence_kind":"source_excerpt","locator":"Lines 174-180","start_line":174,"end_line":180,"excerpt":"## cla-plc-block\nA phospholipase-sensitive step linked membrane signaling to calcium.\nPLC inhibitor U73122 attenuated CLA-induced calcium accumulation, inflammatory signaling and impaired glucose uptake.\nModel: Human adipocyte inhibitor experiment.\nLimitations: Broad inhibitor evidence does not isolate PLC-gamma1 as necessary.\nEvidence access: Primary abstract\nThe phospholipase C inhibitor U73122 attenuates trans-10, cis-12 conjugated linoleic acid-mediated inflammatory signaling and insulin resistance in human adipocytes. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23468551/ · DOI 10.3945/jn.112.173161","model_system":"Human adipocyte inhibitor experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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