{"id":"3bee2a16-04a3-5c0e-9d93-270864477eb1","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-cht1-uptake","predicate":"mediates","statement":"Human CHT1 expression produced high-affinity choline uptake in Xenopus oocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d4401ff6-1141-5264-a2ac-72ad7ccc9e97","mechanism_event_label":"This transporter supplies choline for the neuronal recycling route.","subject":{"id":"04646285-c618-515d-8baa-57f7805cf6c0","slug":"slc5a7","display_name":"Human high-affinity choline transporter CHT1 / SLC5A7","entity_type_key":"protein"},"object":{"id":"698875f9-8800-5abc-ba9c-9b6fcc2628b2","slug":"human-cht1-choline-uptake","display_name":"Human CHT1-mediated choline uptake in expression assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d4401ff6-1141-5264-a2ac-72ad7ccc9e97","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-cht1-uptake-event","event_type":"biochemical_relationship","label":"This transporter supplies choline for the neuronal recycling route.","description":"Human CHT1 expression produced high-affinity choline uptake in Xenopus oocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8e1d19fd-ad66-575d-81c4-9122f78915e5","slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"04646285-c618-515d-8baa-57f7805cf6c0","slug":"slc5a7","display_name":"Human high-affinity choline transporter CHT1 / SLC5A7","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"698875f9-8800-5abc-ba9c-9b6fcc2628b2","slug":"human-cht1-choline-uptake","display_name":"Human CHT1-mediated choline uptake in expression assays","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/11068039.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f9885c7f9525456b2c9eac0865a1f86cda2cf8f26cd6a8e34c212218f3a22bd5\", \"start_char\": 0, \"end_char\": 1088, \"text_sha256\": \"f9885c7f9525456b2c9eac0865a1f86cda2cf8f26cd6a8e34c212218f3a22bd5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human CHT1 expression in Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Choline, sodium and chloride titrations; hemicholinium-3 inhibition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay EC50 values and Hill coefficients are not dietary requirements or exact ion-coupling stoichiometry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human transporter; Xenopus oocyte host","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This transporter supplies choline for the neuronal recycling route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p11068039] Functional characterization of the human high-affinity choline transporter. (2000). https://pubmed.ncbi.nlm.nih.gov/11068039/ DOI: 10.1016/s0014-5793(00)02134-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Plasma-membrane transport assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a11a785b-f245-51d6-89c9-ba0cd581929a","evidence_kind":"source_excerpt","locator":"Lines 282-293","start_line":282,"end_line":293,"excerpt":"### choline-cht1-uptake\nHuman CHT1 expression produced high-affinity choline uptake in Xenopus oocytes.\nCondition category: normal\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This transporter supplies choline for the neuronal recycling route.\norganism: Human transporter; Xenopus oocyte host\ntissue_or_cell_type: Plasma-membrane transport assay\nexperimental_model: Human CHT1 expression in Xenopus oocytes\nlimitations: Assay EC50 values and Hill coefficients are not dietary requirements or exact ion-coupling stoichiometry.\nexposure: Choline, sodium and chloride titrations; hemicholinium-3 inhibition\nevidence_span: {\"source_cache\": \"artifacts/choline-research/11068039.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f9885c7f9525456b2c9eac0865a1f86cda2cf8f26cd6a8e34c212218f3a22bd5\", \"start_char\": 0, \"end_char\": 1088, \"text_sha256\": \"f9885c7f9525456b2c9eac0865a1f86cda2cf8f26cd6a8e34c212218f3a22bd5\"}\n[choline-p11068039] Functional characterization of the human high-affinity choline transporter. 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