{"id":"3b3a7550-f64d-5a12-921e-25a2a45ecc63","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-pdxp-mouse-pools","predicate":"increases","statement":"Ubiquitous Pdxp deletion raised PLP in brain, skeletal muscle and red cells by up to threefold compared with controls.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"8aa85c98-1875-5e82-bd1a-e154897d595d","mechanism_event_label":"Slower breakdown increased tissue PLP.","subject":{"id":"5e7258bf-98a9-54b0-b3e1-1040eb62603a","slug":"mouse-pdxp-knockout","display_name":"Mouse Pdxp knockout genotype","entity_type_key":"gene"},"object":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"8aa85c98-1875-5e82-bd1a-e154897d595d","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-pdxp-mouse-pools-event","event_type":"biochemical_relationship","label":"Slower breakdown increased tissue PLP.","description":"Ubiquitous Pdxp deletion raised PLP in brain, skeletal muscle and red cells by up to threefold compared with controls.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7e62c8cf-79e6-5fa0-84b3-4bb5c119be71","slug":"mouse-pdxp","display_name":"Mouse Pdxp pyridoxal phosphatase","entity_type_key":"protein"},"role":"deleted protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5e7258bf-98a9-54b0-b3e1-1040eb62603a","slug":"mouse-pdxp-knockout","display_name":"Mouse Pdxp knockout genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_location","value_text":"Indexed abstract: tissue PLP measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Conditional and ubiquitous Pdxp-deletion mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Genetic Pdxp ablation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Effects vary by tissue; knockout does not model ordinary B6 intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Slower breakdown increased tissue PLP.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[jeanclos2019] Improved cognition, mild anxiety-like behavior and decreased motor performance in pyridoxal phosphatase-deficient mice. (2019). https://pubmed.ncbi.nlm.nih.gov/30327125/ DOI: 10.1016/j.bbadis.2018.08.018","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brain, skeletal muscle and erythrocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"554ba3e6-4d2f-5848-bc62-57b7e22d4446","evidence_kind":"source_excerpt","locator":"Lines 296-307","start_line":296,"end_line":307,"excerpt":"### b6-transport-pdxp-mouse-pools\nUbiquitous Pdxp deletion raised PLP in brain, skeletal muscle and red cells by up to threefold compared with controls.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Slower breakdown increased tissue PLP.\norganism: Mus musculus\ntissue_or_cell_type: Brain, skeletal muscle and erythrocytes\nexperimental_model: Conditional and ubiquitous Pdxp-deletion mice.\nlimitations: Effects vary by tissue; knockout does not model ordinary B6 intake.\nexposure: Genetic Pdxp ablation.\nevidence_location: Indexed abstract: tissue PLP measurements\n[jeanclos2019] Improved cognition, mild anxiety-like behavior and decreased motor performance in pyridoxal phosphatase-deficient mice. 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