{"id":"3b32205c-59b8-54fc-913e-937f3acae88d","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-ape1-aggregation","predicate":"forms","statement":"Myricetin formed aggregates at 0.33 mM and above; 0.05% Brij35 or centrifugation disrupted or removed them.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"230bb0a1-588f-5348-b69b-35c2782c1f48","mechanism_event_label":"The assay solution itself can create an apparent target effect.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"a28b4558-b063-5be1-8c45-ee91aac7cb36","slug":"myricetin-colloidal-aggregates","display_name":"Myricetin colloidal aggregates in APE1 assay buffer","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"230bb0a1-588f-5348-b69b-35c2782c1f48","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-ape1-aggregation-event","event_type":"observed_relationship","label":"The assay solution itself can create an apparent target effect.","description":"Myricetin formed aggregates at 0.33 mM and above; 0.05% Brij35 or centrifugation disrupted or removed them.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a28b4558-b063-5be1-8c45-ee91aac7cb36","slug":"myricetin-colloidal-aggregates","display_name":"Myricetin colloidal aggregates in APE1 assay buffer","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dynamic light scattering under the specified buffer conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Threshold is buffer-specific; not a biological aggregation threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The assay solution itself can create an apparent target effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Characterizing inhibitors of human AP endonuclease 1. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36652434/ · DOI 10.1371/journal.pone.0280526","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6c8b1d7e-d89c-5695-88fb-c9599b55394b","evidence_kind":"source_excerpt","locator":"Lines 668-674","start_line":668,"end_line":674,"excerpt":"## myricetin-ape1-aggregation\nThe assay solution itself can create an apparent target effect.\nMyricetin formed aggregates at 0.33 mM and above; 0.05% Brij35 or centrifugation disrupted or removed them.\nModel: Dynamic light scattering under the specified buffer conditions.\nLimitations: Threshold is buffer-specific; not a biological aggregation threshold.\nEvidence access: Primary full text\nCharacterizing inhibitors of human AP endonuclease 1. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36652434/ · DOI 10.1371/journal.pone.0280526","model_system":"Dynamic light scattering under the specified buffer conditions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0abf512275cf3aae9e4ec8544f1cde97e0155d0cfb5be12a193cdd5a898386a7","revision_id":"422e342f-a55c-5e73-ba98-43df560f49b9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}