{"id":"3afa5e89-1ad0-59f1-bf55-a1a038047a27","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-plc-calcium","predicate":"reduces","statement":"The PLC inhibitor U73122 reduced the intracellular calcium rise and AMPK/ACC phosphorylation after resveratrol in mouse C2C12 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bf1e5dba-7818-523a-b8c4-e5cdd1a4a31b","mechanism_event_label":"A phospholipase-linked step lies upstream of calcium signaling.","subject":{"id":"ffe25d58-72d0-52a5-a83e-f7898f5c3ac3","slug":"u73122","display_name":"U73122, pharmacological PLC inhibitor","entity_type_key":"small_molecule"},"object":{"id":"d8fe50ce-2814-50f9-b05a-ab0214435b4c","slug":"mouse-c2c12-resveratrol-calcium-release","display_name":"Resveratrol-induced calcium increase in mouse C2C12 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"bf1e5dba-7818-523a-b8c4-e5cdd1a4a31b","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-plc-calcium-event","event_type":"observed_relationship","label":"A phospholipase-linked step lies upstream of calcium signaling.","description":"The PLC inhibitor U73122 reduced the intracellular calcium rise and AMPK/ACC phosphorylation after resveratrol in mouse C2C12 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ffe25d58-72d0-52a5-a83e-f7898f5c3ac3","slug":"u73122","display_name":"U73122, pharmacological PLC inhibitor","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d8fe50ce-2814-50f9-b05a-ab0214435b4c","slug":"mouse-c2c12-resveratrol-calcium-release","display_name":"Resveratrol-induced calcium increase in mouse C2C12 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"871e74d6-6e9e-5e9c-9c6b-f269f59cb02a","slug":"mouse-ampk-complexes","display_name":"Mouse AMPK complexes","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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