{"id":"3af34e44-ebd5-5d8c-b189-2f827ff27d35","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-svg-insulin","predicate":"increases","statement":"Steviol glucuronide increased insulin release from isolated mouse islets, maximally at 100 nM with 16.7 mM glucose; enhancement required glucose of at least 11.1 mM in the tested range.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e59c9680-fd26-52d9-a644-39efffc19596","mechanism_event_label":"The conjugated metabolite is not necessarily biologically inactive.","subject":{"id":"f008b74d-164b-5663-a72e-04a48fe7a8fb","slug":"steviol-glucuronide","display_name":"Steviol glucuronide","entity_type_key":"small_molecule"},"object":{"id":"58b41e29-580c-5ea3-a6a3-629c2f358f87","slug":"mouse-svg-insulin-secretion","display_name":"Mouse insulin secretion after steviol glucuronide","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e59c9680-fd26-52d9-a644-39efffc19596","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-svg-insulin-event","event_type":"observed_relationship","label":"The conjugated metabolite is not necessarily biologically inactive.","description":"Steviol glucuronide increased insulin release from isolated mouse islets, maximally at 100 nM with 16.7 mM glucose; enhancement required glucose of at least 11.1 mM in the tested range.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f008b74d-164b-5663-a72e-04a48fe7a8fb","slug":"steviol-glucuronide","display_name":"Steviol glucuronide","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58b41e29-580c-5ea3-a6a3-629c2f358f87","slug":"mouse-svg-insulin-secretion","display_name":"Mouse insulin secretion after steviol glucuronide","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"d7ab69c0-cd7d-587c-b315-47af56493182","slug":"insulin","display_name":"Insulin","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Sixty-minute static incubation and islet perifusion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"TRPM5 mediation was not established in this experiment; human glucose-lowering efficacy is separate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The conjugated metabolite is not necessarily biologically inactive.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Steviol glucuronide, a metabolite of steviol glycosides, potently stimulates insulin secretion from isolated mouse islets: Studies in vitro. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31592450/ · DOI 10.1002/edm2.93","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eaaf4714-dfc8-5af2-9a61-1274a8ea8346","evidence_kind":"source_excerpt","locator":"Lines 250-256","start_line":250,"end_line":256,"excerpt":"## stevia-svg-insulin\nThe conjugated metabolite is not necessarily biologically inactive.\nSteviol glucuronide increased insulin release from isolated mouse islets, maximally at 100 nM with 16.7 mM glucose; enhancement required glucose of at least 11.1 mM in the tested range.\nModel: Sixty-minute static incubation and islet perifusion.\nLimitations: TRPM5 mediation was not established in this experiment; human glucose-lowering efficacy is separate.\nEvidence access: Primary abstract\nSteviol glucuronide, a metabolite of steviol glycosides, potently stimulates insulin secretion from isolated mouse islets: Studies in vitro. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31592450/ · DOI 10.1002/edm2.93","model_system":"Sixty-minute static incubation and islet perifusion.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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