{"id":"3aef5f89-9eb6-50af-9e42-8c008df7bc3d","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:aged-nfkb","predicate":"decreases_in_recorded_experiment","statement":"Thymulin with equimolar zinc reduced I-kappa-B-alpha phosphorylation and p65 DNA binding in conditioned mouse macrophages.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ce2e96f4-93e9-5b56-b393-b26a9a0fb7ed","mechanism_event_label":"Thymulin with equimolar zinc reduced I-kappa-B-alpha phosphorylation and p65 DNA binding in conditioned mouse macrophages.","subject":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"object":{"id":"51ccf7f9-d3b9-5ef7-83ca-78a14399ea34","slug":"mouse-nfkb-pathway","display_name":"Mouse NF-kappa-B signaling readouts","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ce2e96f4-93e9-5b56-b393-b26a9a0fb7ed","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:aged-nfkb-event","event_type":"experimental_observation","label":"Thymulin with equimolar zinc reduced I-kappa-B-alpha phosphorylation and p65 DNA binding in conditioned mouse macrophages.","description":"The 2026 Nature Communications study used **thymulin plus equimolar zinc versus zinc-matched controls**. Human PBMCs received 24-hour treatment before stimulated cytokine assessment. Mouse macrophages were conditioned during a week of differentiation, then challenged with LPS. Reduced IκBα phosphorylation, p65 DNA binding and inflammatory cytokine responses were reported. Mutation of an NF-κB site in an Il6 reporter removed the inhibitory reporter effect.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"tested factor","stoichiometry":null,"state_label":"Thymulin plus equimolar zinc","sequence_order":0,"notes":""},{"entity":{"id":"51ccf7f9-d3b9-5ef7-83ca-78a14399ea34","slug":"mouse-nfkb-pathway","display_name":"Mouse NF-kappa-B signaling readouts","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Full text obtained; relevant results/methods inspected. 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The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. 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