{"id":"3ae36808-082f-54a6-9cf2-4a465722c556","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-calcium-soce","predicate":"modulates","statement":"Sodium butyrate promoted store-operated calcium entry and activated the CaMKK-beta/AMPK pathway during Caco-2 tight-junction reassembly, without the change being explained by ATP concentration.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a65f89a2-cf27-5103-8c85-6f4e82aad87e","mechanism_event_label":"Calcium signaling helped connect butyrate to barrier repair.","subject":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"object":{"id":"826d902e-14df-5677-9e54-5b0988e76f25","slug":"human-butyrate-soce","display_name":"Store-operated calcium entry in butyrate-exposed Caco-2 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a65f89a2-cf27-5103-8c85-6f4e82aad87e","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-calcium-soce-event","event_type":"observed_relationship","label":"Calcium signaling helped connect butyrate to barrier repair.","description":"Sodium butyrate promoted store-operated calcium entry and activated the CaMKK-beta/AMPK pathway during Caco-2 tight-junction reassembly, without the change being explained by ATP concentration.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"826d902e-14df-5677-9e54-5b0988e76f25","slug":"human-butyrate-soce","display_name":"Store-operated calcium entry in butyrate-exposed Caco-2 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"002c9475-0df2-52d4-bbbb-5649661b2340","slug":"camkk2","display_name":"Human calcium/calmodulin-dependent protein kinase kinase 2 / CAMKK2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"95bc303f-ad04-59ea-8e1e-32f9e8b72c56","slug":"human-ampk-complexes","display_name":"Human AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"4baf6011-dcc4-5a69-9c77-a21de6060383","slug":"sodium-butyrate","display_name":"Sodium butyrate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human Caco-2 calcium-switch model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not show that dietary calcium or magnesium supplementation improves the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Calcium signaling helped connect butyrate to barrier repair.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Sodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/MLC2 Pathway and Phosphorylation of PKCβ2. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27735862/ · DOI 10.3390/ijms17101696","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d10143bd-3dc2-5807-9d1f-5a07b84a38ee","evidence_kind":"source_excerpt","locator":"Lines 270-276","start_line":270,"end_line":276,"excerpt":"## butyrate-calcium-soce\nCalcium signaling helped connect butyrate to barrier repair.\nSodium butyrate promoted store-operated calcium entry and activated the CaMKK-beta/AMPK pathway during Caco-2 tight-junction reassembly, without the change being explained by ATP concentration.\nModel: Human Caco-2 calcium-switch model.\nLimitations: This does not show that dietary calcium or magnesium supplementation improves the response.\nEvidence access: Primary abstract\nSodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/MLC2 Pathway and Phosphorylation of PKCβ2. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27735862/ · DOI 10.3390/ijms17101696","model_system":"Human Caco-2 calcium-switch model.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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