{"id":"3a7a93e4-cb71-504d-9856-335e540678e5","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-slc52a2-localization","predicate":"localizes-to","statement":"Tagged human Riboflavin transporter 2 / SLC52A2 localized mainly in intracellular vesicles, with some basolateral expression in polarized Caco-2 and MDCK imaging experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"3bd9b0b1-6d69-59d9-94dd-9cb8b48daf19","mechanism_event_label":"The riboflavin transporters occupy different parts of polarized epithelial cells.","subject":{"id":"67aa820f-85b6-583e-a350-5a09c7b36eea","slug":"slc52a2","display_name":"Riboflavin transporter 2 / SLC52A2","entity_type_key":"protein"},"object":{"id":"76f447fd-e754-5337-ae0b-b8597a99fc8a","slug":"intracellular-vesicles","display_name":"Intracellular vesicles","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"3bd9b0b1-6d69-59d9-94dd-9cb8b48daf19","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-slc52a2-localization-event","event_type":"biochemical_relationship","label":"The riboflavin transporters occupy different parts of polarized epithelial cells.","description":"Tagged human Riboflavin transporter 2 / SLC52A2 localized mainly in intracellular vesicles, with some basolateral expression in polarized Caco-2 and MDCK imaging experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67aa820f-85b6-583e-a350-5a09c7b36eea","slug":"slc52a2","display_name":"Riboflavin transporter 2 / SLC52A2","entity_type_key":"protein"},"role":"localized-protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"76f447fd-e754-5337-ae0b-b8597a99fc8a","slug":"intracellular-vesicles","display_name":"Intracellular vesicles","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Live-cell imaging; historical RFT1/2/3 correspond to RFVT1/3/2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Live-cell confocal imaging of tagged transporter constructs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Expression of fluorescent transporter constructs.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Tagged overexpression in epithelial models; not a universal localization map across tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human proteins in human and canine cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The riboflavin transporters occupy different parts of polarized epithelial cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[transport-localization-2011] Differential expression of human riboflavin transporters -1, -2, and -3 in polarized epithelia: a key role for hRFT-2 in intestinal riboflavin uptake. (2011). https://pubmed.ncbi.nlm.nih.gov/21854757/ DOI: 10.1016/j.bbamem.2011.08.004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Polarized Caco-2 and MDCK cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"94b41e6a-f79c-5c9b-88d0-9e1974d85b56","evidence_kind":"source_excerpt","locator":"Lines 189-200","start_line":189,"end_line":200,"excerpt":"### transport-slc52a2-localization\nTagged human Riboflavin transporter 2 / SLC52A2 localized mainly in intracellular vesicles, with some basolateral expression in polarized Caco-2 and MDCK imaging experiments.\nCondition category: normal\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The riboflavin transporters occupy different parts of polarized epithelial cells.\norganism: Human proteins in human and canine cells\ntissue_or_cell_type: Polarized Caco-2 and MDCK cells\nexperimental_model: Live-cell confocal imaging of tagged transporter constructs\nlimitations: Tagged overexpression in epithelial models; not a universal localization map across tissues.\nexposure: Expression of fluorescent transporter constructs.\nevidence_location: Live-cell imaging; historical RFT1/2/3 correspond to RFVT1/3/2\n[transport-localization-2011] Differential expression of human riboflavin transporters -1, -2, and -3 in polarized epithelia: a key role for hRFT-2 in intestinal riboflavin uptake. 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