{"id":"3a3d7772-49be-5814-b642-dde29bae9df3","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-slc52a1-localization","predicate":"localizes-to","statement":"Tagged human Riboflavin transporter 1 / SLC52A1 localized mainly at the basolateral membrane in polarized Caco-2 and MDCK imaging experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"a52b652f-ed14-5d04-8c4e-a0932ab73952","mechanism_event_label":"The riboflavin transporters occupy different parts of polarized epithelial cells.","subject":{"id":"109b2e78-a275-58bc-88a7-44d77ea85046","slug":"slc52a1","display_name":"Riboflavin transporter 1 / SLC52A1","entity_type_key":"protein"},"object":{"id":"1c3dfab8-3b5e-569a-9bea-90535ed288e2","slug":"intestinal-epithelial-basolateral-membrane","display_name":"Intestinal epithelial basolateral membrane","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"a52b652f-ed14-5d04-8c4e-a0932ab73952","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-slc52a1-localization-event","event_type":"biochemical_relationship","label":"The riboflavin transporters occupy different parts of polarized epithelial cells.","description":"Tagged human Riboflavin transporter 1 / SLC52A1 localized mainly at the basolateral membrane in polarized Caco-2 and MDCK imaging experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"109b2e78-a275-58bc-88a7-44d77ea85046","slug":"slc52a1","display_name":"Riboflavin transporter 1 / SLC52A1","entity_type_key":"protein"},"role":"localized-protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1c3dfab8-3b5e-569a-9bea-90535ed288e2","slug":"intestinal-epithelial-basolateral-membrane","display_name":"Intestinal epithelial basolateral membrane","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Live-cell imaging; historical RFT1/2/3 correspond to RFVT1/3/2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Live-cell confocal imaging of tagged transporter constructs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Expression of fluorescent transporter constructs.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Tagged overexpression in epithelial models; not a universal localization map across tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human proteins in human and canine cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The riboflavin transporters occupy different parts of polarized epithelial cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[transport-localization-2011] Differential expression of human riboflavin transporters -1, -2, and -3 in polarized epithelia: a key role for hRFT-2 in intestinal riboflavin uptake. (2011). https://pubmed.ncbi.nlm.nih.gov/21854757/ DOI: 10.1016/j.bbamem.2011.08.004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Polarized Caco-2 and MDCK cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e02000e2-348b-5893-bc17-6e8cc00c7895","evidence_kind":"source_excerpt","locator":"Lines 176-187","start_line":176,"end_line":187,"excerpt":"### transport-slc52a1-localization\nTagged human Riboflavin transporter 1 / SLC52A1 localized mainly at the basolateral membrane in polarized Caco-2 and MDCK imaging experiments.\nCondition category: normal\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The riboflavin transporters occupy different parts of polarized epithelial cells.\norganism: Human proteins in human and canine cells\ntissue_or_cell_type: Polarized Caco-2 and MDCK cells\nexperimental_model: Live-cell confocal imaging of tagged transporter constructs\nlimitations: Tagged overexpression in epithelial models; not a universal localization map across tissues.\nexposure: Expression of fluorescent transporter constructs.\nevidence_location: Live-cell imaging; historical RFT1/2/3 correspond to RFVT1/3/2\n[transport-localization-2011] Differential expression of human riboflavin transporters -1, -2, and -3 in polarized epithelia: a key role for hRFT-2 in intestinal riboflavin uptake. 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