{"id":"3a1430f8-f587-5e1b-895a-c81623494340","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-aasdhppt-mtacp","predicate":"donates_prosthetic_group_to","statement":"Human AASDHPPT transfers the CoA-derived 4′-phosphopantetheine group to a conserved serine in human mitochondrial ACP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"17103257-6cc3-55e2-8d83-0f5d118006fe","mechanism_event_label":"The mitochondrial carrier protein also needs a chemical arm derived from CoA.","subject":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"object":{"id":"f8b8ea84-05ad-5cdc-97a0-bfa19e40560c","slug":"ndufab1-holo","display_name":"Human phosphopantetheinylated mitochondrial ACP","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"17103257-6cc3-55e2-8d83-0f5d118006fe","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-aasdhppt-mtacp-event","event_type":"biochemical_relationship","label":"The mitochondrial carrier protein also needs a chemical arm derived from CoA.","description":"Human AASDHPPT transfers the CoA-derived 4′-phosphopantetheine group to a conserved serine in human mitochondrial ACP.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"df1ba694-d4ab-5a09-adac-272f9f9df60d","slug":"aasdhppt","display_name":"Human 4-phosphopantetheinyl transferase / AASDHPPT","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2c0bfd00-1cde-5542-82e6-527d9b72908c","slug":"ndufab1","display_name":"Human mitochondrial acyl carrier protein / NDUFAB1","entity_type_key":"protein"},"role":"acceptor_protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"aab78666-4f68-5db2-b86e-a3c6b18ed62d","slug":"phosphopantetheine","display_name":"4′-Phosphopantetheine","entity_type_key":"small_molecule"},"role":"transferred_moiety","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f8b8ea84-05ad-5cdc-97a0-bfa19e40560c","slug":"ndufab1-holo","display_name":"Human phosphopantetheinylated mitochondrial ACP","entity_type_key":"protein_state"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Abstract, substrate-specificity result","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human transferase and mitochondrial ACP substrate","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Enzymatic transfer from CoA; no dietary intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The experiment establishes substrate competence, not the in-vivo location of the transfer reaction. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The mitochondrial carrier protein also needs a chemical arm derived from CoA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-joshi2003] Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity. (2003). https://pubmed.ncbi.nlm.nih.gov/12815048/ DOI: 10.1074/jbc.m305459200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial ACP substrate; in-vitro reaction","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"041f47ff-6980-5039-9b72-fa6518fa4bfb","evidence_kind":"source_excerpt","locator":"Lines 809-821","start_line":809,"end_line":821,"excerpt":"### b5-met-aasdhppt-mtacp\nHuman AASDHPPT transfers the CoA-derived 4′-phosphopantetheine group to a conserved serine in human mitochondrial ACP.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The mitochondrial carrier protein also needs a chemical arm derived from CoA.\norganism: Homo sapiens\ntissue_or_cell_type: Mitochondrial ACP substrate; in-vitro reaction\nexperimental_model: Recombinant human transferase and mitochondrial ACP substrate\nlimitations: The experiment establishes substrate competence, not the in-vivo location of the transfer reaction. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Enzymatic transfer from CoA; no dietary intervention.\ncross_nutrient: false\nevidence_location: Abstract, substrate-specificity result\n[b5-met-joshi2003] Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity. (2003). https://pubmed.ncbi.nlm.nih.gov/12815048/ DOI: 10.1074/jbc.m305459200","model_system":"Recombinant human transferase and mitochondrial ACP substrate","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-joshi2003] Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity. 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