{"id":"3a0cae3f-4dd3-5608-a1fb-632fa19c49b0","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-mpo-activation","predicate":"oxidizes","statement":"Human myeloperoxidase with hydrogen peroxide oxidized eugenol toward a reactive intermediate consistent with a quinone methide.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"68b05101-13de-54f8-b48f-8e0d9a9ca5e9","mechanism_event_label":"Human myeloperoxidase with hydrogen peroxide oxidized eugenol toward a reactive intermediate consistent with a quinone methide.","subject":{"id":"53f51828-4ea1-5f2e-a044-e02577272436","slug":"mpo","display_name":"Human myeloperoxidase / MPO","entity_type_key":"protein"},"object":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"68b05101-13de-54f8-b48f-8e0d9a9ca5e9","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-mpo-activation-event","event_type":"observed_relationship","label":"Human myeloperoxidase with hydrogen peroxide oxidized eugenol toward a reactive intermediate consistent with a quinone methide.","description":"Human myeloperoxidase with hydrogen peroxide oxidized eugenol toward a reactive intermediate consistent with a quinone methide.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53f51828-4ea1-5f2e-a044-e02577272436","slug":"mpo","display_name":"Human myeloperoxidase / MPO","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d67cd5c6-7764-5cd5-a43b-5b368f2cb441","slug":"eugenol-quinone-methide","display_name":"Eugenol quinone-methide intermediate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Eugenol with peroxidase and hydrogen peroxide","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human MPO and activated human polymorphonuclear leukocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The reactive intermediate was inferred from chemistry; ordinary dietary exposure and organ-specific dose were not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Purified human MPO and activated human polymorphonuclear leukocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Human myeloperoxidase with hydrogen peroxide oxidized eugenol toward a reactive intermediate consistent with a quinone methide.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic activation of eugenol by myeloperoxidase and polymorphonuclear leukocytes. (1989). https://pubmed.ncbi.nlm.nih.gov/2562421/ DOI: 10.1021/tx00009a011","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Reactive-metabolite formation and protein binding","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d8e86eef-7517-5672-90a3-85840a9c810e","evidence_kind":"source_excerpt","locator":"Lines 88-97","start_line":88,"end_line":97,"excerpt":"## eugenol-mpo-activation\nHuman myeloperoxidase with hydrogen peroxide oxidized eugenol toward a reactive intermediate consistent with a quinone methide.\nModel/species: Purified human MPO and activated human polymorphonuclear leukocytes\nTissue/system: Reactive-metabolite formation and protein binding\nExposure: Eugenol with peroxidase and hydrogen peroxide\nRoute: In vitro\nDuration: Acute\nLimits: The reactive intermediate was inferred from chemistry; ordinary dietary exposure and organ-specific dose were not established.\nPrimary reference: Metabolic activation of eugenol by myeloperoxidase and polymorphonuclear leukocytes. (1989). https://pubmed.ncbi.nlm.nih.gov/2562421/ DOI: 10.1021/tx00009a011\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Purified human MPO and activated human polymorphonuclear leukocytes","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"28c421c41a2fbfb00f3036084d43389247cfbfbad8f7549b7529fb4f0e84f61d","revision_id":"3377bcaa-7d21-522a-8571-50ff1977aba0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}