{"id":"39f7713a-efa3-55bb-8b4e-d6aea706a287","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-beta-glucose-flux","predicate":"increases","statement":"Adding 10 mM alanine to 16.7 mM glucose increased glucose utilization about 2.4-fold in BRIN-BD11 cells and increased measured oxidative and nonoxidative metabolism.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a498a9e9-2dc8-525d-bc37-1077ac76fa12","mechanism_event_label":"Alanine changed how the cultured cell processed glucose.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"e11e0a4e-f80a-557f-9ade-5aa3e9f2d20c","slug":"rat-brin-glucose-utilization","display_name":"Glucose utilization in rat BRIN-BD11 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a498a9e9-2dc8-525d-bc37-1077ac76fa12","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-beta-glucose-flux-event","event_type":"observed_relationship","label":"Alanine changed how the cultured cell processed glucose.","description":"Adding 10 mM alanine to 16.7 mM glucose increased glucose utilization about 2.4-fold in BRIN-BD11 cells and increased measured oxidative and nonoxidative metabolism.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e11e0a4e-f80a-557f-9ade-5aa3e9f2d20c","slug":"rat-brin-glucose-utilization","display_name":"Glucose utilization in rat BRIN-BD11 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat clonal beta-cell NMR experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Millimolar culture exposures; normal rat islets show different responses under other conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Alanine changed how the cultured cell processed glucose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A nuclear magnetic resonance-based demonstration of substantial oxidative L-alanine metabolism and L-alanine-enhanced glucose metabolism in a clonal pancreatic beta-cell line: metabolism of L-alanine is important to the regulation of insulin secretion. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12031957/ · DOI 10.2337/diabetes.51.6.1714","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6fa5335b-bc6e-51bf-9a3c-ed47e969b4ab","evidence_kind":"source_excerpt","locator":"Lines 368-374","start_line":368,"end_line":374,"excerpt":"## alanine-beta-glucose-flux\nAlanine changed how the cultured cell processed glucose.\nAdding 10 mM alanine to 16.7 mM glucose increased glucose utilization about 2.4-fold in BRIN-BD11 cells and increased measured oxidative and nonoxidative metabolism.\nModel: Rat clonal beta-cell NMR experiments.\nLimitations: Millimolar culture exposures; normal rat islets show different responses under other conditions.\nEvidence access: Primary abstract\nA nuclear magnetic resonance-based demonstration of substantial oxidative L-alanine metabolism and L-alanine-enhanced glucose metabolism in a clonal pancreatic beta-cell line: metabolism of L-alanine is important to the regulation of insulin secretion. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12031957/ · DOI 10.2337/diabetes.51.6.1714","model_system":"Rat clonal beta-cell NMR experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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