{"id":"39f34e0f-1944-53ce-98a1-89ef993805e5","stable_key":"6a5c5b4f-d859-5f43-ab2f-45c594a570ae:dele1-loss-dfo-atf4","predicate":"has_contextual_experimental_effect","statement":"DELE1 knockout suppressed iron-chelation-induced ATF4 activation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b5a457d9-b5e1-555b-ac0b-3b8f46b99244","mechanism_event_label":"dele1 loss dfo atf4","subject":{"id":"94820007-ca86-5394-8c35-37d2ee3d4f8f","slug":"human-dele1-knockout","display_name":"DELE1 knockout in human cultured cells","entity_type_key":"protein_state"},"object":{"id":"1a9dd2b3-2552-5387-a086-66bea9f56058","slug":"atf4","display_name":"Human activating transcription factor 4 / ATF4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b5a457d9-b5e1-555b-ac0b-3b8f46b99244","stable_key":"6a5c5b4f-d859-5f43-ab2f-45c594a570ae:dele1-loss-dfo-atf4","event_type":"experimental_result","label":"dele1 loss dfo atf4","description":"DELE1 knockout suppressed iron-chelation-induced ATF4 activation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"94820007-ca86-5394-8c35-37d2ee3d4f8f","slug":"human-dele1-knockout","display_name":"DELE1 knockout in human cultured cells","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1a9dd2b3-2552-5387-a086-66bea9f56058","slug":"atf4","display_name":"Human activating transcription factor 4 / ATF4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"279d00c2-a6a3-5943-8d5d-a7eed68e1041","slug":"deferoxamine","display_name":"Deferoxamine","entity_type_key":"drug"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"selected_indexed_full_text_passages","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_cache","value_text":"artifacts/discovery-research/round2-sources/primary-mechanism-passages.json; SHA256 81c0207494f0b2a22fd0af0d70528a5114dfd74367dd99f967f4ada68c53e8c3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HEK293-derived knockout clones 24/51","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"16 hours; DFP 1 mM in its arm; DFO concentration unresolved","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Selected indexed primary full-text passages reviewed; supplements not independently inspected in this round. 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