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(2016). https://pubmed.ncbi.nlm.nih.gov/27729528/ DOI: 10.1073/pnas.1608679113","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Naïve embryonic stem cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e3c8a1da-55d0-53f7-927c-47f755da9833","evidence_kind":"source_excerpt","locator":"Lines 1047-1059","start_line":1047,"end_line":1059,"excerpt":"### c-reg-retinol-tet2-expression\nRetinol supplementation increased Tet2 transcription in mouse naïve ESCs within 8 h and after 72 h; Tet3 also responded, whereas evidence did not support direct retinol stimulation of TET catalytic efficiency.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin A raised expression of DNA-modifying enzymes through a different route from vitamin C’s iron-redox effect.\norganism: Mus musculus\ntissue_or_cell_type: Naïve embryonic stem cells\nexperimental_model: Mouse naïve ESCs in vitamin-A-free N2B27/2i medium; transcript quantification\nlimitations: Retinoid signaling and response depend on culture context; no claim that vitamin A supplementation demethylates adult human tissues.\nexposure: Retinol titration 0–50 ng/mL; 8-h and 72-h transcript assays.\ncross_nutrient: true\nevidence_location: Figure 3A–C\n[c-reg-hore] Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. 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