{"id":"3906f0a3-5a19-5bbe-b4a2-52696bcdbcc9","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-mouse-atp-dose","predicate":"changes_with_dose","statement":"In mouse C2C12 cells, 25 micromolar resveratrol increased ATP at later time points, while 50 micromolar reduced ATP and mitochondrial membrane potential.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6360eaf4-e22e-51b9-8535-24153b81d7a2","mechanism_event_label":"AMPK activation can accompany energy stress rather than improved energy supply.","subject":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"object":{"id":"72cb2836-cd4c-534e-86f5-18c140717a14","slug":"mouse-c2c12-atp-content","display_name":"ATP content in mouse C2C12 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6360eaf4-e22e-51b9-8535-24153b81d7a2","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-mouse-atp-dose-event","event_type":"observed_relationship","label":"AMPK activation can accompany energy stress rather than improved energy supply.","description":"In mouse C2C12 cells, 25 micromolar resveratrol increased ATP at later time points, while 50 micromolar reduced ATP and mitochondrial membrane potential.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"72cb2836-cd4c-534e-86f5-18c140717a14","slug":"mouse-c2c12-atp-content","display_name":"ATP content in mouse C2C12 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dose and time-course experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Context difference is recorded directly, not labelled an unexplained contradiction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"AMPK activation can accompany energy stress rather than improved energy supply.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"SIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22560220/ · DOI 10.1016/j.cmet.2012.04.003","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5d9c4bc2-eca2-5fae-bf6a-30a24eb7eca2","evidence_kind":"source_excerpt","locator":"Lines 190-196","start_line":190,"end_line":196,"excerpt":"## resveratrol-mouse-atp-dose\nAMPK activation can accompany energy stress rather than improved energy supply.\nIn mouse C2C12 cells, 25 micromolar resveratrol increased ATP at later time points, while 50 micromolar reduced ATP and mitochondrial membrane potential.\nModel: Dose and time-course experiments.\nLimitations: Context difference is recorded directly, not labelled an unexplained contradiction.\nEvidence access: Primary full text\nSIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22560220/ · DOI 10.1016/j.cmet.2012.04.003","model_system":"Dose and time-course experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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