{"id":"38c1f489-1775-54cf-9bab-8213b1f2f84d","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-anion-exchanger-block","predicate":"inhibitor_attenuates","statement":"DIDS inhibited the intracellular thiol decrease and extracellular increase during vanadate exposure, supporting an anion-transport-dependent step.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a0773c9e-65cf-59b1-891a-f8c362bfdbcf","mechanism_event_label":"Membrane transport can gate the redox response.","subject":{"id":"803d4606-8b52-5019-8fc1-724b3b635015","slug":"slc4a1","display_name":"Human erythrocyte anion exchanger 1 / SLC4A1","entity_type_key":"protein"},"object":{"id":"1ad15cf2-df32-5ea0-83f3-30317902aab7","slug":"human-erythrocyte-vanadate-gsh-efflux","display_name":"Glutathione efflux from vanadate-exposed human erythrocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a0773c9e-65cf-59b1-891a-f8c362bfdbcf","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-anion-exchanger-block-event","event_type":"observed_relationship","label":"Membrane transport can gate the redox response.","description":"DIDS inhibited the intracellular thiol decrease and extracellular increase during vanadate exposure, supporting an anion-transport-dependent step.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"803d4606-8b52-5019-8fc1-724b3b635015","slug":"slc4a1","display_name":"Human erythrocyte anion exchanger 1 / SLC4A1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1ad15cf2-df32-5ea0-83f3-30317902aab7","slug":"human-erythrocyte-vanadate-gsh-efflux","display_name":"Glutathione efflux from vanadate-exposed human erythrocytes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"b28dfc54-a5ef-5f9e-ac1f-890b68e58dc3","slug":"inorganic-phosphate","display_name":"Inorganic phosphate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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