{"id":"389ca179-00c1-521e-9ea9-ab3505372767","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-metal-sensitive-hydrolysis","predicate":"inhibits_tested","statement":"1,10-Phenanthroline or amastatin inhibited membrane aspartame metabolism by more than 78%.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a78d949b-2117-59d9-af48-eea8dfb61cc9","mechanism_event_label":"Metal-sensitive peptidase activity is a digestive gate.","subject":{"id":"42673ebe-16e8-5a92-94d7-832449c40238","slug":"1-10-phenanthroline","display_name":"1,10-Phenanthroline","entity_type_key":"small_molecule"},"object":{"id":"164d6ac3-0038-5071-b0dc-d6774ef52141","slug":"human-intestinal-aspartame-hydrolysis","display_name":"Human intestinal microvillar aspartame hydrolysis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a78d949b-2117-59d9-af48-eea8dfb61cc9","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-metal-sensitive-hydrolysis-event","event_type":"observed_relationship","label":"Metal-sensitive peptidase activity is a digestive gate.","description":"1,10-Phenanthroline or amastatin inhibited membrane aspartame metabolism by more than 78%.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"42673ebe-16e8-5a92-94d7-832449c40238","slug":"1-10-phenanthroline","display_name":"1,10-Phenanthroline","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"164d6ac3-0038-5071-b0dc-d6774ef52141","slug":"human-intestinal-aspartame-hydrolysis","display_name":"Human intestinal microvillar aspartame hydrolysis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6e964e46-4708-534b-a643-901f4372037b","slug":"human-enpep","display_name":"Human aminopeptidase A / ENPEP","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human and pig membrane inhibitor assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Inhibitor patterns support aminopeptidase A involvement, not an exclusive gene assignment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Metal-sensitive peptidase activity is a digestive gate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolism of aspartame by human and pig intestinal microvillar peptidases. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8141778/ · DOI 10.1042/bj2980635","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"16d13724-d1d9-5695-8370-2a45384a3165","evidence_kind":"source_excerpt","locator":"Lines 58-64","start_line":58,"end_line":64,"excerpt":"## aspartame-metal-sensitive-hydrolysis\nMetal-sensitive peptidase activity is a digestive gate.\n1,10-Phenanthroline or amastatin inhibited membrane aspartame metabolism by more than 78%.\nModel: Human and pig membrane inhibitor assays.\nLimitations: Inhibitor patterns support aminopeptidase A involvement, not an exclusive gene assignment.\nEvidence access: Primary abstract\nMetabolism of aspartame by human and pig intestinal microvillar peptidases. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8141778/ · DOI 10.1042/bj2980635","model_system":"Human and pig membrane inhibitor assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"21cfc6808062c6ea1fb5d34128bcd005d31f0f6c0d63484794238db77d32ae97","revision_id":"1255dda8-df99-5d4b-a65b-4144c47d7d9b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}