{"id":"387cdd34-07a8-5521-8b54-0af448f516e7","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-coq-feedback","predicate":"inhibits_in_reconstruction","statement":"Excess CoQ suppressed intermediate binding and the promoting effect of COQ8 in the reconstructed system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"45ffb0cb-3d29-5eb7-aaf2-5f957f7a9652","mechanism_event_label":"The finished product can feed back on its own synthesis machinery.","subject":{"id":"ae9544c3-df78-54f8-80e7-5b643e5ba1cf","slug":"ubiquinone","display_name":"Ubiquinone","entity_type_key":"small_molecule"},"object":{"id":"e45b9de8-075b-50b8-87ff-c613836b30f8","slug":"coq-intermediate-delivery","display_name":"Delivery of CoQ biosynthetic intermediates to synthesis enzymes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"45ffb0cb-3d29-5eb7-aaf2-5f957f7a9652","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-coq-feedback-event","event_type":"biochemical_relationship","label":"The finished product can feed back on its own synthesis machinery.","description":"Excess CoQ suppressed intermediate binding and the promoting effect of COQ8 in the reconstructed system.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7439d0d8-d592-5d03-aa7c-e59a57331a70","slug":"tet-coq8b","display_name":"Reconstructed ancestral tetrapod COQ8B","entity_type_key":"protein"},"role":"studied_helper","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ae9544c3-df78-54f8-80e7-5b643e5ba1cf","slug":"ubiquinone","display_name":"Ubiquinone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e45b9de8-075b-50b8-87ff-c613836b30f8","slug":"coq-intermediate-delivery","display_name":"Delivery of CoQ biosynthetic intermediates to synthesis enzymes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coq10-research/42525751.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b7a49b25f09323a11c782afbdbff069e95429ed28e0ce2da5519abcbaa76c632\", \"start_char\": 0, \"end_char\": 1058, \"text_sha256\": \"b7a49b25f09323a11c782afbdbff069e95429ed28e0ce2da5519abcbaa76c632\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reconstructed-protein biochemistry, crystallography and mutagenesis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ATP-dependent pocket gating; excess final CoQ product","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"2026 reconstructed system using short-chain intermediates; do not assign all kinetics directly to intact human mitochondria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coq10","display_name":"Coenzyme Q10 / CoQ10 redox system","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Ancestral tetrapod COQ8A/COQ8B and COQ metabolon","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The finished product can feed back on its own synthesis machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coq10-p42525751] COQ8 chaperones coenzyme Q lipid intermediates through ATP-driven structural gating. (2026). https://pubmed.ncbi.nlm.nih.gov/42525751/ DOI: 10.1126/sciadv.aeg1124","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Lipid-intermediate delivery","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9e3596ed-04a2-525a-834f-348510c18fb9","evidence_kind":"source_excerpt","locator":"Lines 372-383","start_line":372,"end_line":383,"excerpt":"### coq10-coq-feedback\nExcess CoQ suppressed intermediate binding and the promoting effect of COQ8 in the reconstructed system.\nCondition category: normal\nnutrient_topic: Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The finished product can feed back on its own synthesis machinery.\norganism: Ancestral tetrapod COQ8A/COQ8B and COQ metabolon\ntissue_or_cell_type: Lipid-intermediate delivery\nexperimental_model: Reconstructed-protein biochemistry, crystallography and mutagenesis\nlimitations: 2026 reconstructed system using short-chain intermediates; do not assign all kinetics directly to intact human mitochondria.\nexposure: ATP-dependent pocket gating; excess final CoQ product\nevidence_span: {\"source_cache\": \"artifacts/coq10-research/42525751.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b7a49b25f09323a11c782afbdbff069e95429ed28e0ce2da5519abcbaa76c632\", \"start_char\": 0, \"end_char\": 1058, \"text_sha256\": \"b7a49b25f09323a11c782afbdbff069e95429ed28e0ce2da5519abcbaa76c632\"}\n[coq10-p42525751] COQ8 chaperones coenzyme Q lipid intermediates through ATP-driven structural gating. (2026). https://pubmed.ncbi.nlm.nih.gov/42525751/ DOI: 10.1126/sciadv.aeg1124","model_system":"Reconstructed-protein biochemistry, crystallography and mutagenesis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [coq10-p42525751] COQ8 chaperones coenzyme Q lipid intermediates through ATP-driven structural gating. (2026). https://pubmed.ncbi.nlm.nih.gov/42525751/ DOI: 10.1126/sciadv.aeg1124","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fc6c2f3b-14a3-59de-848e-ca1c02fee1df","stable_key":"import-3b5aff9b-4086-5574-bfb4-3ea49ba520d7","title":"Coenzyme Q10: biosynthesis, electron transfer, antioxidant recycling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"053f9a6f6c17321fa9fd271486d7f20a86a49b237424108de7dc8663372e73bf","revision_id":"028900ce-8ca4-5d13-8c86-6e327c071e64","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}