{"id":"38755896-6358-599c-bfb4-fc5b67c6e8a8","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-ampk-required-glut4","predicate":"required_for_protection_of","statement":"AMPK silencing abolished chromium picolinate’s protection against impaired GLUT4 regulation in hyperinsulinemic L6 myotubes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a8d91f9e-9a79-55f9-bd7f-c7c842cbfd90","mechanism_event_label":"When AMPK was depleted, this transporter response to chromium picolinate was lost.","subject":{"id":"378e97ad-f6dc-5b66-a889-e95543049489","slug":"rat-ampk-complexes","display_name":"Rat AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"object":{"id":"46b41076-0f11-5f51-ae03-8441820cefab","slug":"rat-glut4-surface-translocation","display_name":"Rat GLUT4 translocation to the cell surface","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a8d91f9e-9a79-55f9-bd7f-c7c842cbfd90","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-ampk-required-glut4-event","event_type":"biochemical_relationship","label":"When AMPK was depleted, this transporter response to chromium picolinate was lost.","description":"AMPK silencing abolished chromium picolinate’s protection against impaired GLUT4 regulation in hyperinsulinemic L6 myotubes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"89d5d39c-2663-5e0c-8eae-562a5b25a47d","slug":"chromium-iii-picolinate","display_name":"Chromium(III) picolinate / CrPic","entity_type_key":"chemical_species"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b355feba-1bd3-55d6-9f12-54b9fbadfd58","slug":"rat-slc2a4","display_name":"Rat glucose transporter 4 / Slc2a4","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"378e97ad-f6dc-5b66-a889-e95543049489","slug":"rat-ampk-complexes","display_name":"Rat AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"46b41076-0f11-5f51-ae03-8441820cefab","slug":"rat-glut4-surface-translocation","display_name":"Rat GLUT4 translocation to the cell surface","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chromium-research/24725432.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\", \"start_char\": 0, \"end_char\": 1788, \"text_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Hyperinsulinemia model with AMPK silencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Chromium picolinate during chronic low-dose insulin exposure, followed by acute insulin response; AMPK siRNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-culture mechanism. The indexed abstract identifies AMPK depletion without resolving the targeted catalytic isoform; family-level identity is retained. This is not a dietary repletion or human treatment experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chromium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chromium","display_name":"Chromium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rat L6 skeletal muscle myotubes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"When AMPK was depleted, this transporter response to chromium picolinate was lost.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chromium-p24725432] Chromium enhances insulin responsiveness via AMPK. 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The indexed abstract identifies AMPK depletion without resolving the targeted catalytic isoform; family-level identity is retained. This is not a dietary repletion or human treatment experiment.\nexposure: Chromium picolinate during chronic low-dose insulin exposure, followed by acute insulin response; AMPK siRNA\nevidence_span: {\"source_cache\": \"artifacts/chromium-research/24725432.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\", \"start_char\": 0, \"end_char\": 1788, \"text_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\"}\n[chromium-p24725432] Chromium enhances insulin responsiveness via AMPK. 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