{"id":"3872cc1c-f1cd-50f3-8b27-4a3e28e88469","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-rat-gsh-metabolism","predicate":"forms_detected_metabolite","statement":"Rat cucurbitacin B metabolism profiling identified glutathione conjugation among the phase-II routes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e44871c3-d3b9-528d-b638-52105450d4e4","mechanism_event_label":"The existing glutathione node connects chemical interception to metabolism.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"bb5ad406-5c88-5f08-9858-738ecf4cd585","slug":"cucurbitacin-b-glutathione-conjugate","display_name":"Cucurbitacin B glutathione conjugate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"e44871c3-d3b9-528d-b638-52105450d4e4","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-rat-gsh-metabolism-event","event_type":"observed_relationship","label":"The existing glutathione node connects chemical interception to metabolism.","description":"Rat cucurbitacin B metabolism profiling identified glutathione conjugation among the phase-II routes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bb5ad406-5c88-5f08-9858-738ecf4cd585","slug":"cucurbitacin-b-glutathione-conjugate","display_name":"Cucurbitacin B glutathione conjugate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat LC-MS study; also hydrolysis, reduction, epoxidation, amination and other conjugation routes reported.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No human mass balance or obligatory single detoxification pathway was established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The existing glutathione node connects chemical interception to metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic fate of the natural anticancer agent cucurbitacin B: an LC-MS/MS-enabled profiling of its major phase I and II conjugates in vivo. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39441433/ · DOI 10.1007/s00216-024-05608-y","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"06fb680a-0ec0-5584-a9c3-096015c1460d","evidence_kind":"source_excerpt","locator":"Lines 372-378","start_line":372,"end_line":378,"excerpt":"## cucurbitacin-b-rat-gsh-metabolism\nThe existing glutathione node connects chemical interception to metabolism.\nRat cucurbitacin B metabolism profiling identified glutathione conjugation among the phase-II routes.\nModel: Rat LC-MS study; also hydrolysis, reduction, epoxidation, amination and other conjugation routes reported.\nLimitations: No human mass balance or obligatory single detoxification pathway was established.\nEvidence access: Primary abstract\nMetabolic fate of the natural anticancer agent cucurbitacin B: an LC-MS/MS-enabled profiling of its major phase I and II conjugates in vivo. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39441433/ · DOI 10.1007/s00216-024-05608-y","model_system":"Rat LC-MS study; also hydrolysis, reduction, epoxidation, amination and other conjugation routes reported.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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