{"id":"386abf1d-4211-585b-a00a-a6c1c836dec9","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-fungal-membrane","predicate":"disrupts","statement":"CHD-FA exposure increased membrane permeability in Candida albicans assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"081b08db-8cd7-5355-a14d-0747e31d483f","mechanism_event_label":"The tested antifungal action involved a leaky membrane.","subject":{"id":"5184fd06-5a8e-5e9d-99ec-c2feaa59ccde","slug":"carbohydrate-derived-fulvic-acid","display_name":"Carbohydrate-derived fulvic acid / CHD-FA (preparation class)","entity_type_key":"chemical_species"},"object":{"id":"25e259ee-2839-55ce-9015-878b06e7af88","slug":"candida-albicans-membrane-integrity","display_name":"Candida albicans membrane integrity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"081b08db-8cd7-5355-a14d-0747e31d483f","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-fungal-membrane-event","event_type":"observed_relationship","label":"The tested antifungal action involved a leaky membrane.","description":"CHD-FA exposure increased membrane permeability in Candida albicans assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5184fd06-5a8e-5e9d-99ec-c2feaa59ccde","slug":"carbohydrate-derived-fulvic-acid","display_name":"Carbohydrate-derived fulvic acid / CHD-FA (preparation class)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"25e259ee-2839-55ce-9015-878b06e7af88","slug":"candida-albicans-membrane-integrity","display_name":"Candida albicans membrane integrity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Clinical Candida isolate panel; planktonic and biofilm growth; inhibitory concentrations reported as 0.125% and 0.25%.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An in-vitro antiseptic concentration is not a systemic supplement exposure or demonstrated infection treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The tested antifungal action involved a leaky membrane.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carbohydrate Derived Fulvic Acid: An in vitro Investigation of a Novel Membrane Active Antiseptic Agent Against Candida albicans Biofilms. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22479260/ · DOI 10.3389/fmicb.2012.00116","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"df2bd0c2-2b85-5170-8cf3-0685d2c0e7e5","evidence_kind":"source_excerpt","locator":"Lines 124-130","start_line":124,"end_line":130,"excerpt":"## fulvic-acid-fungal-membrane\nThe tested antifungal action involved a leaky membrane.\nCHD-FA exposure increased membrane permeability in Candida albicans assays.\nModel: Clinical Candida isolate panel; planktonic and biofilm growth; inhibitory concentrations reported as 0.125% and 0.25%.\nLimitations: An in-vitro antiseptic concentration is not a systemic supplement exposure or demonstrated infection treatment.\nEvidence access: Primary full text\nCarbohydrate Derived Fulvic Acid: An in vitro Investigation of a Novel Membrane Active Antiseptic Agent Against Candida albicans Biofilms. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22479260/ · DOI 10.3389/fmicb.2012.00116","model_system":"Clinical Candida isolate panel; planktonic and biofilm growth; inhibitory concentrations reported as 0.125% and 0.25%.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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