{"id":"385589ba-8153-5607-9ef9-c7dd4c327332","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-mate1-proton","predicate":"drives","statement":"An inward proton gradient trans-stimulated efflux of intracellular agmatine from MATE1-expressing cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"751ab1f1-0c9c-5f04-b60c-7f4d892f51b1","mechanism_event_label":"The gradient helps determine transport direction.","subject":{"id":"b04f06d7-8de7-55ef-a91c-8be7e143f35d","slug":"inward-proton-gradient-mate1-assay","display_name":"Inward proton gradient in the human MATE1 agmatine assay","entity_type_key":"cellular_process"},"object":{"id":"09197a08-df69-56e2-b892-3f13a67d2c75","slug":"human-mate1-agmatine-efflux","display_name":"Human MATE1-dependent agmatine efflux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"751ab1f1-0c9c-5f04-b60c-7f4d892f51b1","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-mate1-proton-event","event_type":"observed_relationship","label":"The gradient helps determine transport direction.","description":"An inward proton gradient trans-stimulated efflux of intracellular agmatine from MATE1-expressing cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b04f06d7-8de7-55ef-a91c-8be7e143f35d","slug":"inward-proton-gradient-mate1-assay","display_name":"Inward proton gradient in the human MATE1 agmatine assay","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"09197a08-df69-56e2-b892-3f13a67d2c75","slug":"human-mate1-agmatine-efflux","display_name":"Human MATE1-dependent agmatine efflux","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"198b7bfb-7e85-5c91-94b7-32603452a8e3","slug":"slc47a1","display_name":"Human multidrug and toxin extrusion protein 1 / MATE1 / SLC47A1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human MATE1 expression; radiotracer efflux experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not prescribe changing systemic pH.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The gradient helps determine transport direction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"OCT2 and MATE1 provide bidirectional agmatine transport. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21128598/ · DOI 10.1021/mp100180a","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f322ab0c-0058-5037-8459-1fbb6a3b0996","evidence_kind":"source_excerpt","locator":"Lines 44-50","start_line":44,"end_line":50,"excerpt":"## agmatine-sulfate-mate1-proton\nThe gradient helps determine transport direction.\nAn inward proton gradient trans-stimulated efflux of intracellular agmatine from MATE1-expressing cells.\nModel: Human MATE1 expression; radiotracer efflux experiment.\nLimitations: This does not prescribe changing systemic pH.\nEvidence access: Primary abstract\nOCT2 and MATE1 provide bidirectional agmatine transport. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21128598/ · DOI 10.1021/mp100180a","model_system":"Human MATE1 expression; radiotracer efflux experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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