{"id":"3834ab4f-8d17-5781-a2ff-31f2062eaa63","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:milk-glycation-muscle-synthesis-null","predicate":"did_not_differentiate_measured","statement":"Six-hour postexercise muscle protein synthesis showed no significant difference among high-glycation milk protein plus 2 g free leucine, low-glycation milk protein plus 2 g free leucine, and noncaloric placebo groups (P=0.939).","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b45431ce-6d5d-557f-90c5-53b3b6be1c41","mechanism_event_label":"A changed blood nutrient measurement did not translate into a detectable synthesis difference in this experiment.","subject":{"id":"093fc6e3-eaa4-5c1d-bf95-09f4aa30a48f","slug":"glycated-milk-protein","display_name":"Glycated milk protein","entity_type_key":"protein_set"},"object":{"id":"5c40a051-f8ca-50fb-80d3-ed0cd3b29f28","slug":"muscle-protein-synthesis","display_name":"Muscle protein synthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b45431ce-6d5d-557f-90c5-53b3b6be1c41","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:milk-glycation-muscle-synthesis-null-event","event_type":"observed_intervention","label":"A changed blood nutrient measurement did not translate into a detectable synthesis difference in this experiment.","description":"Six-hour postexercise muscle protein synthesis showed no significant difference among high-glycation milk protein plus 2 g free leucine, low-glycation milk protein plus 2 g free leucine, and noncaloric placebo groups (P=0.939).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"093fc6e3-eaa4-5c1d-bf95-09f4aa30a48f","slug":"glycated-milk-protein","display_name":"Glycated milk protein","entity_type_key":"protein_set"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5c40a051-f8ca-50fb-80d3-ed0cd3b29f28","slug":"muscle-protein-synthesis","display_name":"Muscle protein synthesis","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"role":"protein_arm_cointervention","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Randomized parallel trial in 45 healthy young men comparing 20 g high-glycation milk protein plus 2 g free leucine, 20 g low-glycation milk protein plus 2 g free leucine, and noncaloric placebo","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Both protein arms included 2 g free leucine. Acute muscle synthesis rates were 0.059, 0.061, and 0.061 percent per hour for low-glycation, high-glycation, and placebo groups respectively; no significant treatment separation (P=0.939). This null is not proof of equivalence or long-term irrelevance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A changed blood nutrient measurement did not translate into a detectable synthesis difference in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vanlieshout2025] Milk Protein Glycation Compromises Postprandial Lysine Bioavailability but does not Modulate Postprandial Muscle Protein Synthesis Rates In Vivo in Males: A Double-blind, Randomized Parallel Trial (2025). https://pmc.ncbi.nlm.nih.gov/articles/PMC12308134/ DOI: 10.1016/j.tjnut.2025.05.032","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Skeletal muscle","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"faca077c-0a3d-5ec8-b4aa-671905983f65","evidence_kind":"source_excerpt","locator":"Lines 809-817","start_line":809,"end_line":817,"excerpt":"### milk-glycation-muscle-synthesis-null\nSix-hour postexercise muscle protein synthesis showed no significant difference among high-glycation milk protein plus 2 g free leucine, low-glycation milk protein plus 2 g free leucine, and noncaloric placebo groups (P=0.939).\nPlain language: A changed blood nutrient measurement did not translate into a detectable synthesis difference in this experiment.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Skeletal muscle\nexperimental_model: Randomized parallel trial in 45 healthy young men comparing 20 g high-glycation milk protein plus 2 g free leucine, 20 g low-glycation milk protein plus 2 g free leucine, and noncaloric placebo\nlimitations: Both protein arms included 2 g free leucine. Acute muscle synthesis rates were 0.059, 0.061, and 0.061 percent per hour for low-glycation, high-glycation, and placebo groups respectively; no significant treatment separation (P=0.939). This null is not proof of equivalence or long-term irrelevance.\n[vanlieshout2025] Milk Protein Glycation Compromises Postprandial Lysine Bioavailability but does not Modulate Postprandial Muscle Protein Synthesis Rates In Vivo in Males: A Double-blind, Randomized Parallel Trial (2025). https://pmc.ncbi.nlm.nih.gov/articles/PMC12308134/ DOI: 10.1016/j.tjnut.2025.05.032","model_system":"Randomized parallel trial in 45 healthy young men comparing 20 g high-glycation milk protein plus 2 g free leucine, 20 g low-glycation milk protein plus 2 g free leucine, and noncaloric placebo","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [vanlieshout2025] Milk Protein Glycation Compromises Postprandial Lysine Bioavailability but does not Modulate Postprandial Muscle Protein Synthesis Rates In Vivo in Males: A Double-blind, Randomized Parallel Trial (2025). https://pmc.ncbi.nlm.nih.gov/articles/PMC12308134/ DOI: 10.1016/j.tjnut.2025.05.032","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[{"id":"1cbea799-f97b-5af6-8f09-e76e17ff7650","title":"Reduced lysine availability did not predict the measured muscle response","kind":"qualification","status":"open","why":"These are distinct endpoints in the same experiment, not contradictory results. A nutrient-availability surrogate should not be converted into a demonstrated muscle outcome.","resolution":"Unresolved; needs review.","created_at":"2026-09-17 07:46:03","record_type":"conflict","display_label":"Recorded conflict","record_url":"/conflicts/1cbea799-f97b-5af6-8f09-e76e17ff7650","sides":[{"conflict_id":"1cbea799-f97b-5af6-8f09-e76e17ff7650","ordinal":0,"label":"Milk Protein Glycation Compromises Postprandial Lysine Bioavailability but does not Modulate Postprandial Muscle Protein Synthesis Rates In Vivo in Males: A Double-blind, Randomized Parallel Trial","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","start_line":799,"end_line":807,"quote":"### milk-glycation-lysine-availability\nAfter resistance exercise, 20 g highly glycated milk protein yielded lower six-hour plasma lysine availability than less glycated protein in young men; both protein arms also received 2 g free leucine.\nPlain language: Chemical modification of food protein changed how much lysine appeared in circulation.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Blood after feeding\nexperimental_model: Randomized parallel trial in 45 healthy young men comparing 20 g high-glycation milk protein plus 2 g free leucine, 20 g low-glycation milk protein plus 2 g free leucine, and noncaloric placebo\nlimitations: Both protein arms included 2 g free leucine. Processing-related protein modification was manipulated; this was not a free-lysine supplementation or anti-glycation treatment trial.\n[vanlieshout2025] Milk Protein Glycation Compromises Postprandial Lysine Bioavailability but does not Modulate Postprandial Muscle Protein Synthesis Rates In Vivo in Males: A Double-blind, Randomized Parallel Trial (2025). https://pmc.ncbi.nlm.nih.gov/articles/PMC12308134/ DOI: 10.1016/j.tjnut.2025.05.032","source_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","source_title":"L-Lysine: mechanism-first literature curation (2026-09-17)","claim_ids":["6e6530a5-b099-5469-9774-1c1b28bbf6ba"]},{"conflict_id":"1cbea799-f97b-5af6-8f09-e76e17ff7650","ordinal":1,"label":"Milk Protein Glycation Compromises Postprandial Lysine Bioavailability but does not Modulate Postprandial Muscle Protein Synthesis Rates In Vivo in Males: A Double-blind, Randomized Parallel Trial","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","start_line":809,"end_line":817,"quote":"### milk-glycation-muscle-synthesis-null\nSix-hour postexercise muscle protein synthesis showed no significant difference among high-glycation milk protein plus 2 g free leucine, low-glycation milk protein plus 2 g free leucine, and noncaloric placebo groups (P=0.939).\nPlain language: A changed blood nutrient measurement did not translate into a detectable synthesis difference in this experiment.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Skeletal muscle\nexperimental_model: Randomized parallel trial in 45 healthy young men comparing 20 g high-glycation milk protein plus 2 g free leucine, 20 g low-glycation milk protein plus 2 g free leucine, and noncaloric placebo\nlimitations: Both protein arms included 2 g free leucine. Acute muscle synthesis rates were 0.059, 0.061, and 0.061 percent per hour for low-glycation, high-glycation, and placebo groups respectively; no significant treatment separation (P=0.939). This null is not proof of equivalence or long-term irrelevance.\n[vanlieshout2025] Milk Protein Glycation Compromises Postprandial Lysine Bioavailability but does not Modulate Postprandial Muscle Protein Synthesis Rates In Vivo in Males: A Double-blind, Randomized Parallel Trial (2025). https://pmc.ncbi.nlm.nih.gov/articles/PMC12308134/ DOI: 10.1016/j.tjnut.2025.05.032","source_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","source_title":"L-Lysine: mechanism-first literature curation (2026-09-17)","claim_ids":["3834ab4f-8d17-5781-a2ff-31f2062eaa63"]}]}],"corrections":[],"research":null}