{"id":"3825cfba-1c1b-5b0d-9e82-51f65b5869a2","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-wnt-transcription-complex","predicate":"with_smad3_promotes","statement":"Tregs promoted NFAT1-SMAD3 complex assembly in mouse CD8 cells, driving Wnt10b expression in the butyrate-associated bone pathway.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7724426f-823d-5bd8-8869-f2442027d19a","mechanism_event_label":"Two transcription factors linked the immune relay to a bone-building ligand.","subject":{"id":"591314f6-92bd-5ab1-806b-89b3c3bd25ab","slug":"mouse-nfatc2","display_name":"Mouse NFAT1 / Nfatc2","entity_type_key":"protein"},"object":{"id":"24f2ec5a-b6dd-5e40-bd54-b5650827ef07","slug":"mouse-wnt10b","display_name":"Mouse Wnt family member 10B / Wnt10b","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7724426f-823d-5bd8-8869-f2442027d19a","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-wnt-transcription-complex-event","event_type":"observed_relationship","label":"Two transcription factors linked the immune relay to a bone-building ligand.","description":"Tregs promoted NFAT1-SMAD3 complex assembly in mouse CD8 cells, driving Wnt10b expression in the butyrate-associated bone pathway.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"591314f6-92bd-5ab1-806b-89b3c3bd25ab","slug":"mouse-nfatc2","display_name":"Mouse NFAT1 / Nfatc2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"24f2ec5a-b6dd-5e40-bd54-b5650827ef07","slug":"mouse-wnt10b","display_name":"Mouse Wnt family member 10B / Wnt10b","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f28397b4-c673-5943-b836-4ed2ab5d7608","slug":"mouse-smad3","display_name":"Mouse SMAD3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse CD8/Treg mechanistic experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"NFAT1 is Nfatc2, not the osteoclast NFATc1 protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Two transcription factors linked the immune relay to a bone-building ligand.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30446387/ · DOI 10.1016/j.immuni.2018.10.013","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"af4c39ed-b1fe-50ff-b73f-dde641252765","evidence_kind":"source_excerpt","locator":"Lines 462-468","start_line":462,"end_line":468,"excerpt":"## butyrate-wnt-transcription-complex\nTwo transcription factors linked the immune relay to a bone-building ligand.\nTregs promoted NFAT1-SMAD3 complex assembly in mouse CD8 cells, driving Wnt10b expression in the butyrate-associated bone pathway.\nModel: Mouse CD8/Treg mechanistic experiments.\nLimitations: NFAT1 is Nfatc2, not the osteoclast NFATc1 protein.\nEvidence access: Primary abstract\nThe Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30446387/ · DOI 10.1016/j.immuni.2018.10.013","model_system":"Mouse CD8/Treg mechanistic experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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