{"id":"3814c3e4-f5be-53c6-a2b8-32924a99eed4","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:colgalt1-galactosylation","predicate":"galactosylates","statement":"COLGALT1 transfers galactose from UDP-galactose to collagen hydroxylysine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2a50f3c7-3aee-5b5c-8fd8-0b0396bb61f1","mechanism_event_label":"A sugar is attached to a lysine residue that has already been hydroxylated.","subject":{"id":"4dbb65a6-60cb-5fad-b3eb-73ee7038381d","slug":"colgalt1","display_name":"COLGALT1","entity_type_key":"protein"},"object":{"id":"7c465ab2-233e-5401-9706-3492e9ea8f45","slug":"collagen-hydroxylysine-residues","display_name":"Collagen-bound 5-hydroxylysine residues","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"2a50f3c7-3aee-5b5c-8fd8-0b0396bb61f1","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:colgalt1-galactosylation-event","event_type":"biochemical_relationship","label":"A sugar is attached to a lysine residue that has already been hydroxylated.","description":"COLGALT1 transfers galactose from UDP-galactose to collagen hydroxylysine.","status":"provisional","compartment":{"slug":"endoplasmic-reticulum","display_name":"Endoplasmic reticulum"},"participants":[{"entity":{"id":"4dbb65a6-60cb-5fad-b3eb-73ee7038381d","slug":"colgalt1","display_name":"COLGALT1","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7c465ab2-233e-5401-9706-3492e9ea8f45","slug":"collagen-hydroxylysine-residues","display_name":"Collagen-bound 5-hydroxylysine residues","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a76bbd5b-08c9-5d74-8b6f-d69502ffa655","slug":"udp-galactose","display_name":"UDP-galactose","entity_type_key":"small_molecule"},"role":"donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9f832d1d-027d-51c7-945a-c07e27f474bc","slug":"udp","display_name":"UDP","entity_type_key":"small_molecule"},"role":"coproduct","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9e4e923d-b82b-5f01-a370-ec4ca4309246","slug":"collagen-galactosyl-hydroxylysine","display_name":"Collagen galactosyl-hydroxylysine residues","entity_type_key":"protein_state"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Recombinant human COLGALT1 with collagen peptides.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This reaction modifies lysine already in a protein. It does not show that extra oral lysine increases the reaction or improves a clinical outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A sugar is attached to a lysine residue that has already been hydroxylated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[colgalt1-2025] Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1 (2025). https://www.nature.com/articles/s41467-025-59017-5 DOI: 10.1038/s41467-025-59017-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Not specified as a whole tissue; see experimental model.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"99edc3a8-c95b-5ffe-96cc-70d8852c5fce","evidence_kind":"source_excerpt","locator":"Lines 437-445","start_line":437,"end_line":445,"excerpt":"### colgalt1-galactosylation\nCOLGALT1 transfers galactose from UDP-galactose to collagen hydroxylysine.\nPlain language: A sugar is attached to a lysine residue that has already been hydroxylated.\nCondition category: normal\norganism: Human\ntissue_or_cell_type: Not specified as a whole tissue; see experimental model.\nexperimental_model: Recombinant human COLGALT1 with collagen peptides.\nlimitations: This reaction modifies lysine already in a protein. It does not show that extra oral lysine increases the reaction or improves a clinical outcome.\n[colgalt1-2025] Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1 (2025). https://www.nature.com/articles/s41467-025-59017-5 DOI: 10.1038/s41467-025-59017-5","model_system":"Recombinant human COLGALT1 with collagen peptides.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [colgalt1-2025] Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1 (2025). https://www.nature.com/articles/s41467-025-59017-5 DOI: 10.1038/s41467-025-59017-5","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}