{"id":"380e5dc4-b840-5c3d-8ba0-493dd1dca150","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-severe-marker-sequence","predicate":"prolonged_restriction_lowered_blood_folate","statement":"In the single-subject study, serum folate became abnormally low by day 219; RBC folate abnormality was reported at day 413 in the abstract/discussion and day 420 in the Results.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"3c201615-3e33-554d-b212-4a2327ae601e","mechanism_event_label":"Different blood compartments showed the shortage at different times.","subject":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"object":{"id":"0f6abef3-19b7-5cd9-b7f6-53a1048c4480","slug":"erythrocyte-folate-concentration","display_name":"Erythrocyte folate concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3c201615-3e33-554d-b212-4a2327ae601e","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-severe-marker-sequence-event","event_type":"observed_intervention","label":"Different blood compartments showed the shortage at different times.","description":"In the single-subject study, serum folate became abnormally low by day 219; RBC folate abnormality was reported at day 413 in the abstract/discussion and day 420 in the Results.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"69fa9bd2-2d5e-5468-b810-496ee7effcfc","slug":"serum-folate-concentration","display_name":"Serum folate concentration","entity_type_key":"cellular_process"},"role":"earlier_marker","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0f6abef3-19b7-5cd9-b7f6-53a1048c4480","slug":"erythrocyte-folate-concentration","display_name":"Erythrocyte folate concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Single initially folate-replete 58-year-old male self-experiment with serial blood assays and marrow examinations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Prolonged severe folate restriction, major energy restriction/weight loss, and multiple other nutrient supplements; replete diet plus folic acid during recovery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay-dependent observations in a highly supplemented, initially replete individual with severe weight loss; these timings are not typical-person predictions. The paper gives inconsistent day counts for the RBC laboratory-limit crossing; both locations are retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Different blood compartments showed the shortage at different times.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fol-golding2014] Severe experimental folate deficiency in a human subject - a longitudinal study of biochemical and haematological responses as megaloblastic anaemia develops (2014). https://pubmed.ncbi.nlm.nih.gov/25332850/ DOI: 10.1186/2193-1801-3-442","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human blood or whole-person clinical endpoints","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"061f59f0-20c4-56b1-a4e7-f19031b3aafa","evidence_kind":"source_excerpt","locator":"Lines 1505-1515","start_line":1505,"end_line":1515,"excerpt":"### fol-severe-marker-sequence\nIn the single-subject study, serum folate became abnormally low by day 219; RBC folate abnormality was reported at day 413 in the abstract/discussion and day 420 in the Results.\nCondition category: nutrient_deficiency\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Different blood compartments showed the shortage at different times.\norganism: Homo sapiens\ntissue_or_cell_type: Human blood or whole-person clinical endpoints\nexperimental_model: Single initially folate-replete 58-year-old male self-experiment with serial blood assays and marrow examinations.\nlimitations: Assay-dependent observations in a highly supplemented, initially replete individual with severe weight loss; these timings are not typical-person predictions. The paper gives inconsistent day counts for the RBC laboratory-limit crossing; both locations are retained.\nexposure: Prolonged severe folate restriction, major energy restriction/weight loss, and multiple other nutrient supplements; replete diet plus folic acid during recovery.\n[fol-golding2014] Severe experimental folate deficiency in a human subject - a longitudinal study of biochemical and haematological responses as megaloblastic anaemia develops (2014). https://pubmed.ncbi.nlm.nih.gov/25332850/ DOI: 10.1186/2193-1801-3-442","model_system":"Single initially folate-replete 58-year-old male self-experiment with serial blood assays and marrow examinations.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fol-golding2014] Severe experimental folate deficiency in a human subject - a longitudinal study of biochemical and haematological responses as megaloblastic anaemia develops (2014). https://pubmed.ncbi.nlm.nih.gov/25332850/ DOI: 10.1186/2193-1801-3-442","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}