{"id":"37bee4d3-ab3f-5e96-a4ce-898917ebe4ed","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-endothelial-nos3","predicate":"increases_transcript","statement":"Silicon-treated HUVECs increased NOS3 mRNA under the reported normal and peroxide conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7230d58a-39fb-58af-b201-f2d77ac53092","mechanism_event_label":"An individual antioxidant or vascular enzyme was measured.","subject":{"id":"36f2ccf3-5023-5074-aab3-d65d3650b9f6","slug":"metasilicate-derived-culture-solution","display_name":"Sodium-metasilicate-derived soluble silicon culture preparation","entity_type_key":"chemical_species"},"object":{"id":"8a9c57f6-eafd-5243-afaf-d4e830939c4b","slug":"nos3","display_name":"Human endothelial nitric oxide synthase / eNOS / NOS3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7230d58a-39fb-58af-b201-f2d77ac53092","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-endothelial-nos3-event","event_type":"observed_relationship","label":"An individual antioxidant or vascular enzyme was measured.","description":"Silicon-treated HUVECs increased NOS3 mRNA under the reported normal and peroxide conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"36f2ccf3-5023-5074-aab3-d65d3650b9f6","slug":"metasilicate-derived-culture-solution","display_name":"Sodium-metasilicate-derived soluble silicon culture preparation","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8a9c57f6-eafd-5243-afaf-d4e830939c4b","slug":"nos3","display_name":"Human endothelial nitric oxide synthase / eNOS / NOS3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HUVECs, 0.5 mM silicon preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression is not measured catalytic flux or proof that silicon replaces established metal cofactors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"An individual antioxidant or vascular enzyme was measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Ionic silicon improves endothelial cells' survival under toxic oxidative stress by overexpressing angiogenic markers and antioxidant enzymes. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30062712/ · DOI 10.1002/term.2744","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"508836bc-d38c-5158-ad52-0ff455e3ac13","evidence_kind":"source_excerpt","locator":"Lines 312-318","start_line":312,"end_line":318,"excerpt":"## silica-endothelial-nos3\nAn individual antioxidant or vascular enzyme was measured.\nSilicon-treated HUVECs increased NOS3 mRNA under the reported normal and peroxide conditions.\nModel: Human HUVECs, 0.5 mM silicon preparation.\nLimitations: Expression is not measured catalytic flux or proof that silicon replaces established metal cofactors.\nEvidence access: Primary full text\nIonic silicon improves endothelial cells' survival under toxic oxidative stress by overexpressing angiogenic markers and antioxidant enzymes. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30062712/ · DOI 10.1002/term.2744","model_system":"Human HUVECs, 0.5 mM silicon preparation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"e05d81962a1ca6cbfc68fce4fd3dbcdea67c8e61332c19f4fec5d0c3ab8bf1b9","revision_id":"6433ed49-a331-571b-a611-21e56c3ce4bc","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}