{"id":"375fd39f-7b4c-5631-977b-5652d926c056","stable_key":"f2ec2007-88ff-5a96-a73b-8b5085e9cea9:mbz-inhibits-polymerisation","predicate":"inhibits","statement":"Median inhibitory concentrations for nocodazole, oxibendazole, parbendazole, mebendazole and fenbendazole against bovine brain tubulin polymerisation ranged from 1.97 x 10-6 to 6.32 x 10-6 molar, while benomyl, cambendazole and carbendazim had values from 5.83 x 10-5 to 9.01 x 10-5 molar and thiabendazole had a value of 5.49 x 10-4 molar.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"660e0257-d7c2-5c64-a214-ed923b90a0a4","mechanism_event_label":"Mebendazole sits in the potent group; thiabendazole is about a hundred times weaker.","subject":{"id":"f8582a13-fc2e-5127-a61f-5b0594e54069","slug":"mebendazole","display_name":"Mebendazole","entity_type_key":"drug"},"object":{"id":"011e92dc-f29a-5ed1-bc3e-942ffc6664bb","slug":"microtubule-polymerisation","display_name":"Polymerisation of tubulin into microtubules","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"660e0257-d7c2-5c64-a214-ed923b90a0a4","stable_key":"f2ec2007-88ff-5a96-a73b-8b5085e9cea9:mbz-inhibits-polymerisation-event","event_type":"observed_intervention","label":"Mebendazole sits in the potent group; thiabendazole is about a hundred times weaker.","description":"Median inhibitory concentrations for nocodazole, oxibendazole, parbendazole, mebendazole and fenbendazole against bovine brain tubulin polymerisation ranged from 1.97 x 10-6 to 6.32 x 10-6 molar, while benomyl, cambendazole and carbendazim had values from 5.83 x 10-5 to 9.01 x 10-5 molar and thiabendazole had a value of 5.49 x 10-4 molar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3394b04b-6dc0-528b-8e94-0644d5d33f45","slug":"mammalian-brain-tubulin","display_name":"Mammalian brain tubulin","entity_type_key":"protein"},"role":"target_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9119ba51-2ce8-5b18-9fc0-4eaf294b3229","slug":"nocodazole","display_name":"Nocodazole","entity_type_key":"chemical_species"},"role":"comparator","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c557d26e-4eee-5ce2-aef1-8cef5e87fe5e","slug":"thiabendazole","display_name":"Thiabendazole","entity_type_key":"drug"},"role":"weak_comparator","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d67b7610-905f-5e26-bdde-06d7fc12d8e8","slug":"carbendazim","display_name":"Carbendazim","entity_type_key":"chemical_species"},"role":"weak_comparator","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"77f3f912-582a-5144-99ab-f66afe1bb548","slug":"fenbendazole","display_name":"Fenbendazole","entity_type_key":"drug"},"role":"comparator","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"0a658e22-de1d-5ce3-aa3d-31a12faefa1b","slug":"parbendazole","display_name":"Parbendazole","entity_type_key":"drug"},"role":"comparator","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"f8582a13-fc2e-5127-a61f-5b0594e54069","slug":"mebendazole","display_name":"Mebendazole","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""},{"entity":{"id":"011e92dc-f29a-5ed1-bc3e-942ffc6664bb","slug":"microtubule-polymerisation","display_name":"Polymerisation of tubulin into microtubules","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":7,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/mebendazole-research/728472.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"544c36409218c93ebff76d932a2bc6e14ccf7fd6e217d4614c4b561281c2555a\", \"start_char\": 0, \"end_char\": 1025, \"text_sha256\": \"544c36409218c93ebff76d932a2bc6e14ccf7fd6e217d4614c4b561281c2555a\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Turbidimetric microtubule polymerisation assay and colchicine binding assay on purified bovine brain tubulin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Eleven benzimidazoles compared for inhibition of the initial rate of microtubule polymerisation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified mammalian tubulin, which is the off-target rather than the parasite target. It fixes where mebendazole sits in the class by potency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mebendazole research collection; topical membership is not evidence of a direct clinical effect, and mebendazole is recorded separately from albendazole, from the benzimidazole class and from its own crystal forms.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mebendazole","display_name":"Mebendazole","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Cattle","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mebendazole sits in the potent group; thiabendazole is about a hundred times weaker.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mbz-p728472] Interaction of anthelmintic benzimidazoles and benzimidazole derivatives with bovine brain tubulin. 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It fixes where mebendazole sits in the class by potency.\nexposure: Eleven benzimidazoles compared for inhibition of the initial rate of microtubule polymerisation\nevidence_span: {\"source_cache\": \"artifacts/mebendazole-research/728472.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"544c36409218c93ebff76d932a2bc6e14ccf7fd6e217d4614c4b561281c2555a\", \"start_char\": 0, \"end_char\": 1025, \"text_sha256\": \"544c36409218c93ebff76d932a2bc6e14ccf7fd6e217d4614c4b561281c2555a\"}\n[mbz-p728472] Interaction of anthelmintic benzimidazoles and benzimidazole derivatives with bovine brain tubulin. 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