{"id":"374208f7-2b87-5e34-8e5c-798288d1cde4","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:sebocyte-protein","predicate":"decreases_in_recorded_experiment","statement":"The 0.03 wt% mixture lowered the type-I 5α-reductase ELISA signal more than SA alone under testosterone challenge.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"cdbd7aa7-c153-5b80-ba3e-9508a930d0b4","mechanism_event_label":"The 0.03 wt% mixture lowered the type-I 5α-reductase ELISA signal more than SA alone under testosterone challenge.","subject":{"id":"fc69f1ff-9576-5ff1-a6fa-dc5ed9f0390a","slug":"shikimic-acid-prunus-mume-mixture","display_name":"Shikimic acid and Prunus mume extract 1:1 mixture","entity_type_key":"chemical_species"},"object":{"id":"ff60cf2b-7e0b-586c-9197-0888221a4366","slug":"human-sebocyte-srd5a1-elisa","display_name":"Human sebocyte type-I 5-alpha-reductase ELISA amount","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cdbd7aa7-c153-5b80-ba3e-9508a930d0b4","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:sebocyte-protein-event","event_type":"experimental_observation","label":"The 0.03 wt% mixture lowered the type-I 5α-reductase ELISA signal more than SA alone under testosterone challenge.","description":"**A 2026 topical study measured protein amount.** Human sebocytes challenged with 10 nM testosterone were exposed for 72 h to shikimic acid or a 1:1 shikimic-acid/Prunus mume extract mixture. At 0.03 wt%, the mixture lowered the reported type-I 5α-reductase ELISA signal more than shikimic acid alone. The assay measured protein amount in processed supernatant, with three technical replicates and no total-protein normalization; it was not a testosterone-to-DHT catalytic assay. Docking is not measured affinity. Thus a direct 5α-reductase inhibition claim is not justified by this experiment. [Novel prospectives of shikimic acid and Prunus mume extract for enhancing bioactivity on 5α-reductase and stability in cosmetic patches for acne treatment](https://www.frontiersin.org/journals/natural-products/articles/10.3389/fntpr.2025.1737986/full)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fc69f1ff-9576-5ff1-a6fa-dc5ed9f0390a","slug":"shikimic-acid-prunus-mume-mixture","display_name":"Shikimic acid and Prunus mume extract 1:1 mixture","entity_type_key":"chemical_species"},"role":"tested factor","stoichiometry":null,"state_label":"1:1 SA/P. mume mixture plus testosterone","sequence_order":0,"notes":""},{"entity":{"id":"ff60cf2b-7e0b-586c-9197-0888221a4366","slug":"human-sebocyte-srd5a1-elisa","display_name":"Human sebocyte type-I 5-alpha-reductase ELISA amount","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"role":"mixture constituent and comparator","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"919fda15-d9bc-5ed0-a107-c7e94558c9ea","slug":"human-srd5a1","display_name":"Human steroid 5-alpha-reductase type 1 / SRD5A1","entity_type_key":"protein"},"role":"protein assay target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full-text methods/results reviewed. 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No total-protein normalization or independent biological replication established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The 0.03 wt% mixture lowered the type-I 5α-reductase ELISA signal more than SA alone under testosterone challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Novel prospectives of shikimic acid and Prunus mume extract for enhancing bioactivity on 5α-reductase and stability in cosmetic patches for acne treatment | 2026 | DOI 10.3389/fntpr.2025.1737986 | https://www.frontiersin.org/journals/natural-products/articles/10.3389/fntpr.2025.1737986/full","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 79-79; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9525b965-5905-56be-936d-b845d502ed1a","evidence_kind":"source_excerpt","locator":"Lines 79-79","start_line":79,"end_line":79,"excerpt":"**A 2026 topical study measured protein amount.** Human sebocytes challenged with 10 nM testosterone were exposed for 72 h to shikimic acid or a 1:1 shikimic-acid/Prunus mume extract mixture. At 0.03 wt%, the mixture lowered the reported type-I 5α-reductase ELISA signal more than shikimic acid alone. The assay measured protein amount in processed supernatant, with three technical replicates and no total-protein normalization; it was not a testosterone-to-DHT catalytic assay. Docking is not measured affinity. Thus a direct 5α-reductase inhibition claim is not justified by this experiment. [Novel prospectives of shikimic acid and Prunus mume extract for enhancing bioactivity on 5α-reductase and stability in cosmetic patches for acne treatment](https://www.frontiersin.org/journals/natural-products/articles/10.3389/fntpr.2025.1737986/full)","model_system":"Human sebocytes; 10 nM testosterone, 72 h; three technical replicates.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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