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Low-substrate medium is not a validated universal blood threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Making more activation machinery did not guarantee an antimicrobial response when substrate supply was limited.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[adams2009] Vitamin d-directed rheostatic regulation of monocyte antibacterial responses. (2009). https://pubmed.ncbi.nlm.nih.gov/19299728/ DOI: 10.4049/jimmunol.0803736","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human monocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f207db8a-fda1-5688-a2a2-0e6e8f52a2c9","evidence_kind":"source_excerpt","locator":"Lines 983-994","start_line":983,"end_line":994,"excerpt":"### vd-adams-low-substrate\nIn monocytes cultured with 10% autologous serum, 19 kDa TLR2/1 agonist increased CYP27B1 yet reduced hCAP transcript; expression was enhanced by adding 5 nM 25(OH)D3.\nCondition category: nutrient_deficiency\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Making more activation machinery did not guarantee an antimicrobial response when substrate supply was limited.\norganism: Homo sapiens\ntissue_or_cell_type: Human monocytes\nexperimental_model: Human bone-clinic cohort and ex-vivo monocytes in 10% autologous serum\nlimitations: Ex-vivo monocytes in 10% serum, not a whole-person infection endpoint. Low-substrate medium is not a validated universal blood threshold.\nexposure: 24-hour ex-vivo culture; 10% autologous serum, with or without added 5 nM 25(OH)D3.\ncross_nutrient: false\n[adams2009] Vitamin d-directed rheostatic regulation of monocyte antibacterial responses. (2009). https://pubmed.ncbi.nlm.nih.gov/19299728/ DOI: 10.4049/jimmunol.0803736","model_system":"Human bone-clinic cohort and ex-vivo monocytes in 10% autologous serum","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [adams2009] Vitamin d-directed rheostatic regulation of monocyte antibacterial responses. 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