{"id":"3652b8e5-968d-5520-9bc3-9a395b472065","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-cardiac-necrosis","predicate":"induces","statement":"After 24 hours, both solutions caused necrotic cell death without significant caspase-3 activation; approximately 10 micromolar total V produced 50% viability loss.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7d6c493e-9575-523d-a60a-2aec28542cdd","mechanism_event_label":"The injury phenotype was necrotic in this experiment.","subject":{"id":"ab633bfc-d259-5deb-ac81-a3383f79d4bf","slug":"decavanadate","display_name":"Decavanadate(V)","entity_type_key":"chemical_species"},"object":{"id":"82b81739-26ef-5e71-92bd-1517bcf113e0","slug":"rat-cardiomyocyte-vanadate-necrosis","display_name":"Necrotic death in vanadate-exposed rat cardiomyocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7d6c493e-9575-523d-a60a-2aec28542cdd","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-cardiac-necrosis-event","event_type":"observed_relationship","label":"The injury phenotype was necrotic in this experiment.","description":"After 24 hours, both solutions caused necrotic cell death without significant caspase-3 activation; approximately 10 micromolar total V produced 50% viability loss.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab633bfc-d259-5deb-ac81-a3383f79d4bf","slug":"decavanadate","display_name":"Decavanadate(V)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"82b81739-26ef-5e71-92bd-1517bcf113e0","slug":"rat-cardiomyocyte-vanadate-necrosis","display_name":"Necrotic death in vanadate-exposed rat cardiomyocytes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5acecb4f-d4c7-5549-8950-bf6e2085349d","slug":"metavanadate-assay-solution","display_name":"Metavanadate assay solution with mixed oligomers","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"7de13713-9286-548d-8192-102dfd7ef836","slug":"rat-cardiomyocyte-vanadate-depolarization","display_name":"Mitochondrial depolarization in vanadate-exposed neonatal rat cardiomyocytes","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Neonatal rat ventricular cells; MTT and propidium iodide assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No clinical cardiac-risk estimate can be derived from the culture concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"The injury phenotype was necrotic in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Vanadate induces necrotic death in neonatal rat cardiomyocytes through mitochondrial membrane depolarization. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18251508/ · DOI 10.1021/tx700204r","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9ccb17a5-a362-5fc8-bcad-9b9b0055593f","evidence_kind":"source_excerpt","locator":"Lines 294-300","start_line":294,"end_line":300,"excerpt":"## vanadium-cardiac-necrosis\nThe injury phenotype was necrotic in this experiment.\nAfter 24 hours, both solutions caused necrotic cell death without significant caspase-3 activation; approximately 10 micromolar total V produced 50% viability loss.\nModel: Neonatal rat ventricular cells; MTT and propidium iodide assays.\nLimitations: No clinical cardiac-risk estimate can be derived from the culture concentration.\nEvidence access: Primary abstract\nVanadate induces necrotic death in neonatal rat cardiomyocytes through mitochondrial membrane depolarization. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18251508/ · DOI 10.1021/tx700204r","model_system":"Neonatal rat ventricular cells; MTT and propidium iodide assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"d36b30d972e756ca4d19b66f976980e206c65025bd4bbf4c127c17320bd019c2","revision_id":"70d42967-8032-5627-8670-f59841735e89","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}