{"id":"3627c9c0-87f6-53c4-a300-8690a5a9336c","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:dats-gsh","predicate":"reported_relationship","statement":"DATS reacted rapidly with GSH to release sulfide through the reported exchange and perthiol-reduction chemistry.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"608c1b8f-b00f-563e-bab4-24f8f708eeac","mechanism_event_label":"DATS reacted rapidly with GSH to release sulfide through the reported exchange and perthiol-reduction chemistry.","subject":{"id":"267dc359-d357-5af4-9b4a-d4cf3f7360fc","slug":"diallyl-trisulfide","display_name":"Diallyl trisulfide / DATS","entity_type_key":"small_molecule"},"object":{"id":"3dd09568-ca8d-58b3-a147-cdf1aa62e3b0","slug":"dats-gsh-sulfide-production","display_name":"Sulfide generation in the DATS plus GSH chemical assay","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"608c1b8f-b00f-563e-bab4-24f8f708eeac","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:dats-gsh-event","event_type":"biochemical_relationship","label":"DATS reacted rapidly with GSH to release sulfide through the reported exchange and perthiol-reduction chemistry.","description":"**DATS and DADS differ.** A later chemical study found rapid GSH-dependent sulfide release from DATS, involving an allyl-perthiol intermediate, but very little and slow release from DADS. DATS contamination could account for fast release attributed to DADS. Sulfur-chain length, purity, and reaction conditions belong in the record. This qualifies older attribution without deleting either observation; it does not establish that allicin or aged garlic extract has the same behavior. [Liang 2015](https://pubmed.ncbi.nlm.nih.gov/26301500/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"267dc359-d357-5af4-9b4a-d4cf3f7360fc","slug":"diallyl-trisulfide","display_name":"Diallyl trisulfide / DATS","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"3dd09568-ca8d-58b3-a147-cdf1aa62e3b0","slug":"dats-gsh-sulfide-production","display_name":"Sulfide generation in the DATS plus GSH chemical assay","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"reducing thiol","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"70cc3ced-8adc-5e76-9750-e1415683455b","slug":"hydrogen-sulfide","display_name":"Hydrogen sulfide / H2S","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, exact concentrations or species-specific attribution remain unextracted unless explicitly stated in the abstract or separately verified publisher text.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified-compound chemical study; abstract-level access.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"DATS-specific; no automatic transfer to allicin, DADS, aged extract, or human blood-pressure response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"DATS reacted rapidly with GSH to release sulfide through the reported exchange and perthiol-reduction chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Diallyl Trisulfide Is a Fast H2S Donor, but Diallyl Disulfide Is a Slow One: The Reaction Pathways and Intermediates of Glutathione with Polysulfides. | 2015 | DOI 10.1021/acs.orglett.5b01962 | PMID 26301500 | https://pubmed.ncbi.nlm.nih.gov/26301500/ | https://doi.org/10.1021/acs.orglett.5b01962","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 83-83; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7e495e39-0ce7-5334-91bc-05a047f21ebd","evidence_kind":"source_excerpt","locator":"Lines 83-83","start_line":83,"end_line":83,"excerpt":"**DATS and DADS differ.** A later chemical study found rapid GSH-dependent sulfide release from DATS, involving an allyl-perthiol intermediate, but very little and slow release from DADS. DATS contamination could account for fast release attributed to DADS. Sulfur-chain length, purity, and reaction conditions belong in the record. This qualifies older attribution without deleting either observation; it does not establish that allicin or aged garlic extract has the same behavior. [Liang 2015](https://pubmed.ncbi.nlm.nih.gov/26301500/)","model_system":"Purified-compound chemical study; abstract-level access.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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