{"id":"3623740f-a2d6-51fd-a403-1f13ca3a8054","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-opg-blocks-retinoid-calcium-release","predicate":"blocks_retinoid_stimulated","statement":"Exogenous OPG blocked retinoic-acid-stimulated labelled calcium release from calvarial bone.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"386d84ab-395c-5d10-a2ed-7a5280c4624e","mechanism_event_label":"Blocking the RANKL signal interrupted the measured resorption response.","subject":{"id":"02f7fab4-c764-5448-bf91-7b5f6e5f76e5","slug":"tnfrsf11b","display_name":"Osteoprotegerin / TNFRSF11B / OPG","entity_type_key":"protein"},"object":{"id":"0adc6543-b0c1-5a49-9428-80f727ffeb9a","slug":"bone-resorption","display_name":"Osteoclastic bone resorption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"386d84ab-395c-5d10-a2ed-7a5280c4624e","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-opg-blocks-retinoid-calcium-release-event","event_type":"biochemical_relationship","label":"Blocking the RANKL signal interrupted the measured resorption response.","description":"Exogenous OPG blocked retinoic-acid-stimulated labelled calcium release from calvarial bone.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"role":"stimulus","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"de7ad036-388f-596d-8766-72afe51b4a67","slug":"tnfsf11","display_name":"RANK ligand / TNFSF11","entity_type_key":"protein"},"role":"blocked_signal","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"released_tracer","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"02f7fab4-c764-5448-bf91-7b5f6e5f76e5","slug":"tnfrsf11b","display_name":"Osteoprotegerin / TNFRSF11B / OPG","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"0adc6543-b0c1-5a49-9428-80f727ffeb9a","slug":"bone-resorption","display_name":"Osteoclastic bone resorption","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Vitamin A/RANKL -> calcium release.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Same organ culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preparation-specific causal intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Blocking the RANKL signal interrupted the measured resorption response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[va-conaway2011] Retinoids stimulate periosteal bone resorption by enhancing the protein RANKL, a response inhibited by monomeric glucocorticoid receptor (2011). https://pubmed.ncbi.nlm.nih.gov/21715325/ DOI: 10.1074/jbc.m111.247734","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Bone","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6ac694a4-c0c6-58a9-9417-ef804eb09ba3","evidence_kind":"source_excerpt","locator":"Lines 1626-1636","start_line":1626,"end_line":1636,"excerpt":"### va-opg-blocks-retinoid-calcium-release\nExogenous OPG blocked retinoic-acid-stimulated labelled calcium release from calvarial bone.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Blocking the RANKL signal interrupted the measured resorption response.\norganism: Mus musculus\ntissue_or_cell_type: Bone\nexperimental_model: Same organ culture.\nlimitations: Preparation-specific causal intervention.\ncross_nutrient: Vitamin A/RANKL -> calcium release.\n[va-conaway2011] Retinoids stimulate periosteal bone resorption by enhancing the protein RANKL, a response inhibited by monomeric glucocorticoid receptor (2011). https://pubmed.ncbi.nlm.nih.gov/21715325/ DOI: 10.1074/jbc.m111.247734","model_system":"Same organ culture.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [va-conaway2011] Retinoids stimulate periosteal bone resorption by enhancing the protein RANKL, a response inhibited by monomeric glucocorticoid receptor (2011). https://pubmed.ncbi.nlm.nih.gov/21715325/ DOI: 10.1074/jbc.m111.247734","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}