{"id":"35d997a6-dc29-5244-a7f5-8fca6c4c7f6c","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-depletion-increases-hka-activity","predicate":"depletion-increases","statement":"Potassium depletion increased collecting-tubule potassium-stimulated, ouabain-insensitive ATPase activity in rats.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f2e24a46-4a04-5b74-9ccb-f8ab59a9d018","mechanism_event_label":"The distal kidney increased an ATP-consuming activity compatible with potassium recovery.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"77a0b92b-ccdd-5ab2-a032-0ac8fc8f93d5","slug":"renal-hydrogen-potassium-atpases","display_name":"Renal hydrogen-potassium ATPase activities","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"f2e24a46-4a04-5b74-9ccb-f8ab59a9d018","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-depletion-increases-hka-activity-event","event_type":"biochemical_relationship","label":"The distal kidney increased an ATP-consuming activity compatible with potassium recovery.","description":"Potassium depletion increased collecting-tubule potassium-stimulated, ouabain-insensitive ATPase activity in rats.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"role":"upstream","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"77a0b92b-ccdd-5ab2-a032-0ac8fc8f93d5","slug":"renal-hydrogen-potassium-atpases","display_name":"Renal hydrogen-potassium ATPase activities","entity_type_key":"protein_family"},"role":"downstream","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"activating-ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"endpoint","value_text":"Potassium depletion increased collecting-tubule potassium-stimulated, ouabain-insensitive ATPase activity in rats.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Microdissected rabbit nephron segments and collecting tubules of potassium-depleted rats.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Microdissected rabbit nephron segments and collecting tubules of potassium-depleted rats.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"ATP hydrolysis and inhibitor sensitivity establish activity class, not a specific protein isoform or whole-animal flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The distal kidney increased an ATP-consuming activity compatible with potassium recovery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[doucet-1987-hka] Characterization of K-ATPase activity in distal nephron: stimulation by potassium depletion (1987). https://pubmed.ncbi.nlm.nih.gov/2957926/ DOI: 10.1152/ajprenal.1987.253.3.F418","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"collecting tubule","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"3216b661-eed5-589c-a7b0-5eba93b6a4f4","evidence_kind":"source_excerpt","locator":"Lines 1124-1135","start_line":1124,"end_line":1135,"excerpt":"### k-depletion-increases-hka-activity\nPotassium depletion increased collecting-tubule potassium-stimulated, ouabain-insensitive ATPase activity in rats.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The distal kidney increased an ATP-consuming activity compatible with potassium recovery.\norganism: Rattus norvegicus\ntissue_or_cell_type: collecting tubule\nexperimental_model: Microdissected rabbit nephron segments and collecting tubules of potassium-depleted rats.\nlimitations: ATP hydrolysis and inhibitor sensitivity establish activity class, not a specific protein isoform or whole-animal flux.\nexperimental-exposure: Microdissected rabbit nephron segments and collecting tubules of potassium-depleted rats.\nendpoint: Potassium depletion increased collecting-tubule potassium-stimulated, ouabain-insensitive ATPase activity in rats.\n[doucet-1987-hka] Characterization of K-ATPase activity in distal nephron: stimulation by potassium depletion (1987). https://pubmed.ncbi.nlm.nih.gov/2957926/ DOI: 10.1152/ajprenal.1987.253.3.F418","model_system":"Microdissected rabbit nephron segments and collecting tubules of potassium-depleted rats.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [doucet-1987-hka] Characterization of K-ATPase activity in distal nephron: stimulation by potassium depletion (1987). https://pubmed.ncbi.nlm.nih.gov/2957926/ DOI: 10.1152/ajprenal.1987.253.3.F418","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}