{"id":"35d30f0d-fe26-5b1d-b17f-d66ce6427561","stable_key":"d40a4478-67a9-5bdf-b7a2-eb7d17e24dd7:iodine-export-2016-stability","predicate":"increases_protein_stability","statement":"Excess iodide slowed pendrin loss during cycloheximide treatment, supporting increased protein stability.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"2d000989-711c-57c7-ad74-a308792de193","mechanism_event_label":"Excess iodide slowed pendrin loss during cycloheximide treatment, supporting increased protein stability.","subject":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"object":{"id":"3af1ca2d-4042-52fd-ad99-c4a44e5cf2fe","slug":"rat-slc26a4","display_name":"Rat pendrin / Slc26a4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2d000989-711c-57c7-ad74-a308792de193","stable_key":"d40a4478-67a9-5bdf-b7a2-eb7d17e24dd7:iodine-export-2016-stability-event","event_type":"observed_intervention","label":"Excess iodide slowed pendrin loss during cycloheximide treatment, supporting increased protein stability.","description":"Excess iodide slowed pendrin loss during cycloheximide treatment, supporting increased protein stability.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3af1ca2d-4042-52fd-ad99-c4a44e5cf2fe","slug":"rat-slc26a4","display_name":"Rat pendrin / Slc26a4","entity_type_key":"protein"},"role":"measured_target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Rat thyroid PCCl3 cells; 1 mM NaI unless specified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_category","value_text":"Experimental iodide excess; normal is the schema category outside deficiency, machinery impairment and biomarker context.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Nonpolarized cells; concurrent changes do not establish pendrin necessity, human protection, or an intake threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"measurement","value_text":"Cycloheximide chase; Figure 4.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"primary_references","value_text":"Calil-Silveira et al. (2016). Iodide excess regulates its own efflux: a possible involvement of pendrin. DOI: 10.1152/ajpcell.00210.2015. https://pubmed.ncbi.nlm.nih.gov/26791486/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"939f2f9a-1ba9-5fda-8eb9-56e22b917c27","evidence_kind":"source_excerpt","locator":"Lines 17-19","start_line":17,"end_line":19,"excerpt":"## stability\nExcess iodide slowed pendrin loss during cycloheximide treatment, supporting increased protein stability.\nMeasurement: Cycloheximide chase; Figure 4.","model_system":"Rat thyroid PCCl3 cells; 1 mM NaI unless specified.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact curation-document span. Primary study paraphrased; no independent replication implied.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2b88cda3-3556-5f62-b7f5-b12270e6f766","stable_key":"import-d40a4478-67a9-5bdf-b7a2-eb7d17e24dd7","title":"Iodine addendum: iodide excess and cellular export (2026-09-18)","document_type":"imported_text","citation_label":"Calil-Silveira et al. (2016). Iodide excess regulates its own efflux: a possible involvement of pendrin. DOI: 10.1152/ajpcell.00210.2015. https://pubmed.ncbi.nlm.nih.gov/26791486/","file_path":"","sha256":"329cc12a4aaa7d6ccaeca0e5c1ffa3e447ef2debd046fc2bcd9e4f3ae33a13b3","revision_id":"93964ea3-ead3-50bb-ad49-c05a425f73d8","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}