{"id":"35afe4ff-f82b-527f-b15a-c65c2947b08a","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-ischemia-mitochondria","predicate":"reduces","statement":"Carnosine reduced mitochondrial ROS and membrane-potential loss under oxygen-glucose deprivation in astrocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"15e1b4df-d249-5005-90fa-d8bdc1e8f63b","mechanism_event_label":"The cellular response included mitochondrial protection.","subject":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"object":{"id":"2217f746-129a-55f4-8311-c5032e89e7a8","slug":"astrocyte-mitochondrial-ros-species-unresolved","display_name":"Astrocyte mitochondrial ROS under ischemia-like stress, species unresolved","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"15e1b4df-d249-5005-90fa-d8bdc1e8f63b","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-ischemia-mitochondria-event","event_type":"observed_relationship","label":"The cellular response included mitochondrial protection.","description":"Carnosine reduced mitochondrial ROS and membrane-potential loss under oxygen-glucose deprivation in astrocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2217f746-129a-55f4-8311-c5032e89e7a8","slug":"astrocyte-mitochondrial-ros-species-unresolved","display_name":"Astrocyte mitochondrial ROS under ischemia-like stress, species unresolved","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Astrocyte ischemia-like and rotenone experiments; culture species not resolved from the accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Species must be verified before projecting this cellular result onto a species-specific pathway; complete causal mediation was not isolated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The cellular response included mitochondrial protection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carnosine protects against permanent cerebral ischemia in histidine decarboxylase knockout mice by reducing glutamate excitotoxicity. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20043985/ · DOI 10.1016/j.freeradbiomed.2009.12.021","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4d9d0593-fde7-534d-97ed-0f5d6b789188","evidence_kind":"source_excerpt","locator":"Lines 476-482","start_line":476,"end_line":482,"excerpt":"## carnosine-ischemia-mitochondria\nThe cellular response included mitochondrial protection.\nCarnosine reduced mitochondrial ROS and membrane-potential loss under oxygen-glucose deprivation in astrocytes.\nModel: Astrocyte ischemia-like and rotenone experiments; culture species not resolved from the accessed abstract.\nLimitations: Species must be verified before projecting this cellular result onto a species-specific pathway; complete causal mediation was not isolated.\nEvidence access: Primary abstract\nCarnosine protects against permanent cerebral ischemia in histidine decarboxylase knockout mice by reducing glutamate excitotoxicity. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20043985/ · DOI 10.1016/j.freeradbiomed.2009.12.021","model_system":"Astrocyte ischemia-like and rotenone experiments; culture species not resolved from the accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"87dc5af2-4eb6-5980-b7e0-bf3697b1156e","stable_key":"import-ae1ed35d-8ce7-5936-9067-a641374bbf79","title":"Carnosine: synthesis, transport, carbonyl chemistry and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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