{"id":"35a78474-8a9a-5921-8ca7-489b550ecf59","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-mast-release","predicate":"decreases_in_recorded_comparison","statement":"RA reduced histamine and beta-hexosaminidase release in IgE-activated rat RBL-2H3 cells.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"330c1c59-f289-5d5f-a2de-ccb23a56b8bd","mechanism_event_label":"RA reduced histamine and beta-hexosaminidase release in IgE-activated rat RBL-2H3 cells.","subject":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"object":{"id":"3b9de9eb-43f4-5e12-9359-3653b0ec8935","slug":"rat-rbl2h3-degranulation","display_name":"Rat RBL-2H3 degranulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"330c1c59-f289-5d5f-a2de-ccb23a56b8bd","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-mast-release-event","event_type":"experimental_observation","label":"RA reduced histamine and beta-hexosaminidase release in IgE-activated rat RBL-2H3 cells.","description":"### ra-mast-release\n\nRA reduced histamine and beta-hexosaminidase release in IgE-activated rat RBL-2H3 cells.\nNo directly verified Lyn/Syk target is assigned.\nModel and exposure: 2024 RBL-2H3 study; primary abstract, dose/timing not extracted.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Inhibitory effect of rosmarinic acid on IgE-trigged mast cell degranulation in vitro and in vivo. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38270683/ | https://doi.org/10.1007/s11033-023-09164-z | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"RA plus IgE activation","sequence_order":0,"notes":""},{"entity":{"id":"3b9de9eb-43f4-5e12-9359-3653b0ec8935","slug":"rat-rbl2h3-degranulation","display_name":"Rat RBL-2H3 degranulation","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"847daefd-ce13-541a-b426-919975168a3d","slug":"histamine","display_name":"Histamine","entity_type_key":"small_molecule"},"role":"released mediator","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"RA plus IgE activation\", \"comparator\": \"IgE activation without RA\", \"endpoint\": \"RA reduced histamine and beta-hexosaminidase release in IgE-activated rat RBL-2H3 cells.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"2024 RBL-2H3 study; primary abstract, dose/timing not extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"RA reduced histamine and beta-hexosaminidase release in IgE-activated rat RBL-2H3 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibitory effect of rosmarinic acid on IgE-trigged mast cell degranulation in vitro and in vivo. | https://pubmed.ncbi.nlm.nih.gov/38270683/ | https://doi.org/10.1007/s11033-023-09164-z","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-mast-release; No directly verified Lyn/Syk target is assigned.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c648f2f0-68fa-5b82-8153-96eca59b7e59","evidence_kind":"source_excerpt","locator":"Lines 253-259","start_line":253,"end_line":259,"excerpt":"### ra-mast-release\n\nRA reduced histamine and beta-hexosaminidase release in IgE-activated rat RBL-2H3 cells.\nNo directly verified Lyn/Syk target is assigned.\nModel and exposure: 2024 RBL-2H3 study; primary abstract, dose/timing not extracted.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Inhibitory effect of rosmarinic acid on IgE-trigged mast cell degranulation in vitro and in vivo. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38270683/ | https://doi.org/10.1007/s11033-023-09164-z | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"2024 RBL-2H3 study; primary abstract, dose/timing not extracted.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}