{"id":"358a4f14-6d0c-5edd-a153-cf9e6166f50c","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:thiamine-def-human-eaat1-loss","predicate":"deficiency-associated-with-lower","statement":"Frontal-cortex EAAT1 protein was 62% lower in the five Wernicke cases than in five controls.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"6aa9ce33-c59e-576d-85f4-d45c818d8242","mechanism_event_label":"An astrocyte glutamate transporter was reduced in the sampled diseased human cortex.","subject":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"object":{"id":"23bdf240-9ac3-57fc-8270-af878c463361","slug":"slc1a3","display_name":"Human excitatory amino acid transporter 1 / SLC1A3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"6aa9ce33-c59e-576d-85f4-d45c818d8242","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:thiamine-def-human-eaat1-loss-event","event_type":"observed_intervention","label":"An astrocyte glutamate transporter was reduced in the sampled diseased human cortex.","description":"Frontal-cortex EAAT1 protein was 62% lower in the five Wernicke cases than in five controls.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"transporter substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"23bdf240-9ac3-57fc-8270-af878c463361","slug":"slc1a3","display_name":"Human excitatory amino acid transporter 1 / SLC1A3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_location","value_text":"Abstract; Results; Figure 1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"reduced by 62%","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human frontal-cortex autopsy samples: five male Wernicke encephalopathy cases with alcohol intake above 80 g/day versus five male controls below 20 g/day; immunoblotting and histology.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Neuropathologically identified Wernicke encephalopathy with heavy alcohol exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not isolate thiamine from alcohol effects, establish the initiating event, or directly measure uptake in living patients.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An astrocyte glutamate transporter was reduced in the sampled diseased human cortex.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hazell-2010-human-astrocytes] Loss of astrocytic glutamate transporters in Wernicke encephalopathy (2010). https://pmc.ncbi.nlm.nih.gov/articles/PMC3388119/ DOI: 10.1002/glia.20908","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Frontal cortex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"83149cb9-f8ea-58a5-aee3-8f564a3b1eb9","evidence_kind":"source_excerpt","locator":"Lines 1255-1267","start_line":1255,"end_line":1267,"excerpt":"### thiamine-def-human-eaat1-loss\nFrontal-cortex EAAT1 protein was 62% lower in the five Wernicke cases than in five controls.\nCondition category: nutrient_deficiency\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An astrocyte glutamate transporter was reduced in the sampled diseased human cortex.\norganism: Homo sapiens\ntissue_or_cell_type: Frontal cortex\nexperimental_model: Human frontal-cortex autopsy samples: five male Wernicke encephalopathy cases with alcohol intake above 80 g/day versus five male controls below 20 g/day; immunoblotting and histology.\nlimitations: Does not isolate thiamine from alcohol effects, establish the initiating event, or directly measure uptake in living patients.\nevidence_location: Abstract; Results; Figure 1\nevidence_span: reduced by 62%\nexposure: Neuropathologically identified Wernicke encephalopathy with heavy alcohol exposure\n[hazell-2010-human-astrocytes] Loss of astrocytic glutamate transporters in Wernicke encephalopathy (2010). https://pmc.ncbi.nlm.nih.gov/articles/PMC3388119/ DOI: 10.1002/glia.20908","model_system":"Human frontal-cortex autopsy samples: five male Wernicke encephalopathy cases with alcohol intake above 80 g/day versus five male controls below 20 g/day; immunoblotting and histology.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hazell-2010-human-astrocytes] Loss of astrocytic glutamate transporters in Wernicke encephalopathy (2010). https://pmc.ncbi.nlm.nih.gov/articles/PMC3388119/ DOI: 10.1002/glia.20908","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}