{"id":"35779108-93ad-50fc-900a-ad74b69f0ec4","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-zip10-lyn","predicate":"caused_hyperactivation_of","statement":"Reduced CD45R activity in ZIP10-deficient B cells was linked to LYN hyperactivation and dysregulated receptor signaling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d3910930-fadb-5981-8cd5-8a51ed792cbe","mechanism_event_label":"Losing the transporter caused excessive activity at one signaling step rather than simply turning everything down.","subject":{"id":"cf3127a4-a16a-5907-96b4-3273fe4fe379","slug":"mouse-b-cell-zip10-loss","display_name":"Mouse mature-B-cell ZIP10 loss","entity_type_key":"cellular_process"},"object":{"id":"bb00334b-ca17-5ea7-974a-43ce3c530349","slug":"b-cell-lyn-activation","display_name":"B-cell LYN activation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d3910930-fadb-5981-8cd5-8a51ed792cbe","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-zip10-lyn-event","event_type":"biochemical_relationship","label":"Losing the transporter caused excessive activity at one signaling step rather than simply turning everything down.","description":"Reduced CD45R activity in ZIP10-deficient B cells was linked to LYN hyperactivation and dysregulated receptor signaling.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"95f28e8a-7199-565b-9eda-2c0232cbc9c5","slug":"mouse-slc39a10","display_name":"Mouse ZIP10 / Slc39a10","entity_type_key":"protein"},"role":"affected_transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"846decb0-3598-59ca-83c0-cfa6195c772c","slug":"mouse-cd45r","display_name":"Mouse CD45R / Ptprc B-cell isoform","entity_type_key":"protein"},"role":"upstream_phosphatase","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d1df0ee2-8644-5b65-9c31-5c7cbf2b80c3","slug":"mouse-lyn","display_name":"Mouse LYN kinase","entity_type_key":"protein"},"role":"affected_kinase","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"cf3127a4-a16a-5907-96b4-3273fe4fe379","slug":"mouse-b-cell-zip10-loss","display_name":"Mouse mature-B-cell ZIP10 loss","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bb00334b-ca17-5ea7-974a-43ce3c530349","slug":"b-cell-lyn-activation","display_name":"B-cell LYN activation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Mouse ZIP10 / Slc39a10 (affected_transporter); Mouse CD45R / Ptprc B-cell isoform (upstream_phosphatase); Mouse LYN kinase (affected_kinase)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/zinc-signaling-sources/25074919-abstract.txt\", \"locator\": \"Primary indexed abstract\", \"file_sha256\": \"d3032e44cfa64a54cd560b24872d88e69db456a150510b6e4cde64a11e18b4b7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mature-B-cell Zip10-deficient mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"B-cell ZIP10 loss versus controls and receptor-activation comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Genetic loss is distinct from dietary shortage; CD45R/LYN is the study-supported pathway, not a universal direct zinc activation rule for phosphatases.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Losing the transporter caused excessive activity at one signaling step rather than simply turning everything down.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zn-sig-25074919] Zinc transporter SLC39A10/ZIP10 controls humoral immunity by modulating B-cell receptor signal strength. (2014). https://pubmed.ncbi.nlm.nih.gov/25074919/ DOI: 10.1073/pnas.1323557111","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"B cells and antibody responses","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"3ee59531-7fcc-5d45-8458-46bcb429e165","evidence_kind":"source_excerpt","locator":"Lines 1097-1109","start_line":1097,"end_line":1109,"excerpt":"### zn-sig-zip10-lyn\nReduced CD45R activity in ZIP10-deficient B cells was linked to LYN hyperactivation and dysregulated receptor signaling.\nCondition category: machinery_impairment\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Losing the transporter caused excessive activity at one signaling step rather than simply turning everything down.\norganism: Mus musculus\ntissue_or_cell_type: B cells and antibody responses\nexperimental_model: Mature-B-cell Zip10-deficient mice\nlimitations: Genetic loss is distinct from dietary shortage; CD45R/LYN is the study-supported pathway, not a universal direct zinc activation rule for phosphatases.\nexposure: B-cell ZIP10 loss versus controls and receptor-activation comparisons.\ncross_nutrient: Mouse ZIP10 / Slc39a10 (affected_transporter); Mouse CD45R / Ptprc B-cell isoform (upstream_phosphatase); Mouse LYN kinase (affected_kinase)\nevidence_span: {\"source_cache\": \"artifacts/zinc-signaling-sources/25074919-abstract.txt\", \"locator\": \"Primary indexed abstract\", \"file_sha256\": \"d3032e44cfa64a54cd560b24872d88e69db456a150510b6e4cde64a11e18b4b7\"}\n[zn-sig-25074919] Zinc transporter SLC39A10/ZIP10 controls humoral immunity by modulating B-cell receptor signal strength. (2014). https://pubmed.ncbi.nlm.nih.gov/25074919/ DOI: 10.1073/pnas.1323557111","model_system":"Mature-B-cell Zip10-deficient mice","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zn-sig-25074919] Zinc transporter SLC39A10/ZIP10 controls humoral immunity by modulating B-cell receptor signal strength. (2014). https://pubmed.ncbi.nlm.nih.gov/25074919/ DOI: 10.1073/pnas.1323557111","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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