{"id":"352c40da-9713-5b67-9762-3e5f5a42cbf1","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-beta-chronic-calcium","predicate":"reduces_rechallenge_response","statement":"Prior prolonged alanine culture attenuated subsequent alanine-evoked depolarization and intracellular calcium rise in BRIN-BD11 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e9e293da-cb11-556a-bae9-df62ba0aa6ed","mechanism_event_label":"The weakened insulin response was accompanied by weaker electrical and calcium signals.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"95130636-85ca-520f-8fea-9754613f4bcd","slug":"rat-brin-alanine-depolarization-calcium","display_name":"Depolarization and calcium rise after alanine rechallenge","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e9e293da-cb11-556a-bae9-df62ba0aa6ed","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-beta-chronic-calcium-event","event_type":"observed_relationship","label":"The weakened insulin response was accompanied by weaker electrical and calcium signals.","description":"Prior prolonged alanine culture attenuated subsequent alanine-evoked depolarization and intracellular calcium rise in BRIN-BD11 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"95130636-85ca-520f-8fea-9754613f4bcd","slug":"rat-brin-alanine-depolarization-calcium","display_name":"Depolarization and calcium rise after alanine rechallenge","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"430b732c-3469-5fe8-8fde-0686d6900abd","slug":"rat-brin-alanine-insulin","display_name":"Alanine-stimulated insulin secretion in BRIN-BD11 cells","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat clonal beta-cell chronic exposure and acute rechallenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Linked measurements do not prove which upstream molecular change caused desensitization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The weakened insulin response was accompanied by weaker electrical and calcium signals.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Prolonged L-alanine exposure induces changes in metabolism, Ca(2+) handling and desensitization of insulin secretion in clonal pancreatic beta-cells. · 2009 · https://pubmed.ncbi.nlm.nih.gov/18702613/ · DOI 10.1042/CS20080138","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b154757c-0918-56ae-aae1-d9e17466ed61","evidence_kind":"source_excerpt","locator":"Lines 408-414","start_line":408,"end_line":414,"excerpt":"## alanine-beta-chronic-calcium\nThe weakened insulin response was accompanied by weaker electrical and calcium signals.\nPrior prolonged alanine culture attenuated subsequent alanine-evoked depolarization and intracellular calcium rise in BRIN-BD11 cells.\nModel: Rat clonal beta-cell chronic exposure and acute rechallenge.\nLimitations: Linked measurements do not prove which upstream molecular change caused desensitization.\nEvidence access: Primary abstract\nProlonged L-alanine exposure induces changes in metabolism, Ca(2+) handling and desensitization of insulin secretion in clonal pancreatic beta-cells. · 2009 · https://pubmed.ncbi.nlm.nih.gov/18702613/ · DOI 10.1042/CS20080138","model_system":"Rat clonal beta-cell chronic exposure and acute rechallenge.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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