{"id":"34aebcf5-2538-5232-bc65-2d4eb91ea78c","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-snat1-expression","predicate":"increases_expression_of","statement":"Theanine selectively increased Slc38a1 expression in cultured rat neural progenitors.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"94ecb9f7-ae82-5887-8c8e-a819a3d17c6e","mechanism_event_label":"Sustained exposure can change transporter expression, distinct from acute uptake competition.","subject":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"object":{"id":"5e628859-b8d6-5392-a2b6-ba1203e489f7","slug":"rat-slc38a1","display_name":"Rat glutamine transporter SNAT1 / Slc38a1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"94ecb9f7-ae82-5887-8c8e-a819a3d17c6e","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-snat1-expression-event","event_type":"biochemical_relationship","label":"Sustained exposure can change transporter expression, distinct from acute uptake competition.","description":"Theanine selectively increased Slc38a1 expression in cultured rat neural progenitors.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5e628859-b8d6-5392-a2b6-ba1203e489f7","slug":"rat-slc38a1","display_name":"Rat glutamine transporter SNAT1 / Slc38a1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/25957749.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7ebc4fc37f26e19c2d7fb13442e263182e628beda7286839f7d0de0906d59f8e\", \"start_char\": 0, \"end_char\": 1727, \"text_sha256\": \"7ebc4fc37f26e19c2d7fb13442e263182e628beda7286839f7d0de0906d59f8e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Sustained exposure, neurospheres and promoter-reporter experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Theanine 1-100 micromolar; sustained culture exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Immature cells differ from mature neurons/astroglia. Expression changes are not demonstrated neurogenesis in adult humans; reporter experiments do not prove every causal link.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Embryonic rat neural progenitors; mouse P19 reporter cells in separate assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Sustained exposure can change transporter expression, distinct from acute uptake competition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p25957749] Upregulation of Slc38a1 Gene Along with Promotion of Neurosphere Growth and Subsequent Neuronal Specification in Undifferentiated Neural Progenitor Cells Exposed to Theanine. (2016). https://pubmed.ncbi.nlm.nih.gov/25957749/ DOI: 10.1007/s11064-015-1591-4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Progenitor proliferation and lineage commitment","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ac755430-07eb-58ed-be79-d703a687d435","evidence_kind":"source_excerpt","locator":"Lines 328-339","start_line":328,"end_line":339,"excerpt":"### theanine-snat1-expression\nTheanine selectively increased Slc38a1 expression in cultured rat neural progenitors.\nCondition category: normal\nnutrient_topic: L-Theanine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Sustained exposure can change transporter expression, distinct from acute uptake competition.\norganism: Embryonic rat neural progenitors; mouse P19 reporter cells in separate assays\ntissue_or_cell_type: Progenitor proliferation and lineage commitment\nexperimental_model: Sustained exposure, neurospheres and promoter-reporter experiments\nlimitations: Immature cells differ from mature neurons/astroglia. Expression changes are not demonstrated neurogenesis in adult humans; reporter experiments do not prove every causal link.\nexposure: Theanine 1-100 micromolar; sustained culture exposure\nevidence_span: {\"source_cache\": \"artifacts/theanine-research/25957749.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7ebc4fc37f26e19c2d7fb13442e263182e628beda7286839f7d0de0906d59f8e\", \"start_char\": 0, \"end_char\": 1727, \"text_sha256\": \"7ebc4fc37f26e19c2d7fb13442e263182e628beda7286839f7d0de0906d59f8e\"}\n[theanine-p25957749] Upregulation of Slc38a1 Gene Along with Promotion of Neurosphere Growth and Subsequent Neuronal Specification in Undifferentiated Neural Progenitor Cells Exposed to Theanine. (2016). https://pubmed.ncbi.nlm.nih.gov/25957749/ DOI: 10.1007/s11064-015-1591-4","model_system":"Sustained exposure, neurospheres and promoter-reporter experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [theanine-p25957749] Upregulation of Slc38a1 Gene Along with Promotion of Neurosphere Growth and Subsequent Neuronal Specification in Undifferentiated Neural Progenitor Cells Exposed to Theanine. 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