{"id":"34932427-4bfc-59bf-b1da-00546b0a446c","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-human-thiamine-transport","predicate":"transports_with_lower_affinity_than_mouse","statement":"Human OCT1 transported thiamine with approximately 9.5-fold lower affinity than mouse Oct1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"85d66ad0-4377-5551-8b83-ea2fc35c07fc","mechanism_event_label":"Vitamin B1 shares this transporter, but the mouse and human versions behave differently.","subject":{"id":"3cced77c-b044-5d97-8c81-4ff9ed3dd169","slug":"slc22a1","display_name":"Human organic cation transporter 1 / OCT1 / SLC22A1","entity_type_key":"protein"},"object":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"85d66ad0-4377-5551-8b83-ea2fc35c07fc","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-human-thiamine-transport-event","event_type":"biochemical_relationship","label":"Vitamin B1 shares this transporter, but the mouse and human versions behave differently.","description":"Human OCT1 transported thiamine with approximately 9.5-fold lower affinity than mouse Oct1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5b7e7ec2-0310-53bc-b1dd-ad3bac9803cf","slug":"mouse-slc22a1","display_name":"Mouse organic cation transporter 1 / Oct1","entity_type_key":"protein"},"role":"species_comparator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3cced77c-b044-5d97-8c81-4ff9ed3dd169","slug":"slc22a1","display_name":"Human organic cation transporter 1 / OCT1 / SLC22A1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/berberine-research/33037045.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"61d53435dfc952ba419da91767e79c07d75e2a432d9d4a2afd0d8d2560d570fd\", \"start_char\": 0, \"end_char\": 2298, \"text_sha256\": \"61d53435dfc952ba419da91767e79c07d75e2a432d9d4a2afd0d8d2560d570fd\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human versus mouse transporter kinetics in transfected HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Thiamine and metformin transport; transporter chimeras","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human OCT1 affinity for thiamine was substantially lower than mouse Oct1. This is a shared-transporter connection, not proof berberine causes B1 deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Berberine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human OCT1 and mouse Oct1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin B1 shares this transporter, but the mouse and human versions behave differently.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[berberine-p33037045] Differences in Metformin and Thiamine Uptake between Human and Mouse Organic Cation Transporter 1: Structural Determinants and Potential Consequences for Intrahepatic Concentrations. (2020). https://pubmed.ncbi.nlm.nih.gov/33037045/ DOI: 10.1124/dmd.120.000170","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cellular thiamine uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"A species-affinity comparison, but the measurement is thiamine carried into the expressing cell, which stands for the hepatocyte.","entity":null},{"dimension":"transport_pool","value_text":"the hepatocyte interior","comparator":null,"unit":null,"notes":"A species-affinity comparison, but the measurement is thiamine carried into the expressing cell, which stands for the hepatocyte.","entity":null}],"evidence":[{"id":"f733afb8-137f-5822-82d3-f30756cc89a6","evidence_kind":"source_excerpt","locator":"Lines 298-309","start_line":298,"end_line":309,"excerpt":"### berberine-human-thiamine-transport\nHuman OCT1 transported thiamine with approximately 9.5-fold lower affinity than mouse Oct1.\nCondition category: normal\nnutrient_topic: Berberine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin B1 shares this transporter, but the mouse and human versions behave differently.\norganism: Human OCT1 and mouse Oct1\ntissue_or_cell_type: Cellular thiamine uptake\nexperimental_model: Human versus mouse transporter kinetics in transfected HEK293 cells\nlimitations: Human OCT1 affinity for thiamine was substantially lower than mouse Oct1. This is a shared-transporter connection, not proof berberine causes B1 deficiency.\nexposure: Thiamine and metformin transport; transporter chimeras\nevidence_span: {\"source_cache\": \"artifacts/berberine-research/33037045.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"61d53435dfc952ba419da91767e79c07d75e2a432d9d4a2afd0d8d2560d570fd\", \"start_char\": 0, \"end_char\": 2298, \"text_sha256\": \"61d53435dfc952ba419da91767e79c07d75e2a432d9d4a2afd0d8d2560d570fd\"}\n[berberine-p33037045] Differences in Metformin and Thiamine Uptake between Human and Mouse Organic Cation Transporter 1: Structural Determinants and Potential Consequences for Intrahepatic Concentrations. (2020). https://pubmed.ncbi.nlm.nih.gov/33037045/ DOI: 10.1124/dmd.120.000170","model_system":"Human versus mouse transporter kinetics in transfected HEK293 cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [berberine-p33037045] Differences in Metformin and Thiamine Uptake between Human and Mouse Organic Cation Transporter 1: Structural Determinants and Potential Consequences for Intrahepatic Concentrations. (2020). https://pubmed.ncbi.nlm.nih.gov/33037045/ DOI: 10.1124/dmd.120.000170","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9bb61e1d-e5cf-5b05-a9ca-409291f7a313","stable_key":"import-7484635e-cc8f-5cd6-a978-6dc5775a7db5","title":"Berberine: metabolism, nutrient connections and drug interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2f376a035ae79c93e62e2ea4a8d5385643bf16553f108d12b6a80c89e6d41f4c","revision_id":"2a099429-1c4a-55be-aa2c-0565d08bda80","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}