{"id":"34907dd7-6ef9-5cca-81e1-61782e92a09d","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:mid1-depletion-ampk","predicate":"increases_in_recorded_experiment","statement":"MID1IP1 siRNA depletion increased AMPK phosphorylation in HepG2 cells.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"e62dfa91-8a0d-5e8f-b63c-129ff930a8d9","mechanism_event_label":"MID1IP1 siRNA depletion increased AMPK phosphorylation in HepG2 cells.","subject":{"id":"15810693-dac5-5c46-b80b-d57124cd2aae","slug":"human-mid1ip1","display_name":"Human MID1 interacting protein 1 / MID1IP1","entity_type_key":"protein"},"object":{"id":"96985eed-99a7-5eeb-9fc4-0a4956c3ee6f","slug":"human-sa-ampk-phosphorylation","display_name":"Human HepG2 AMPK phosphorylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e62dfa91-8a0d-5e8f-b63c-129ff930a8d9","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:mid1-depletion-ampk-event","event_type":"experimental_observation","label":"MID1IP1 siRNA depletion increased AMPK phosphorylation in HepG2 cells.","description":"**The lipid study contains a useful null result.** Depleting MID1IP1 by siRNA in HepG2 cells reduced SREBP-1c and FAS expression and increased AMPK/ACC phosphorylation. 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[Hypolipogenic Effect of Shikimic Acid Via Inhibition of MID1IP1 and Phosphorylation of AMPK/ACC.](https://pubmed.ncbi.nlm.nih.gov/30700011/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15810693-dac5-5c46-b80b-d57124cd2aae","slug":"human-mid1ip1","display_name":"Human MID1 interacting protein 1 / MID1IP1","entity_type_key":"protein"},"role":"tested factor","stoichiometry":null,"state_label":"MID1IP1 siRNA depletion","sequence_order":0,"notes":""},{"entity":{"id":"96985eed-99a7-5eeb-9fc4-0a4956c3ee6f","slug":"human-sa-ampk-phosphorylation","display_name":"Human HepG2 AMPK phosphorylation","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"MID1IP1 siRNA depletion\", \"comparator\": \"Control transfection\", \"endpoint\": \"MID1IP1 siRNA depletion increased AMPK phosphorylation in HepG2 cells.\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Human HepG2 genetic perturbation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The subject is depleted, not added; this is not an ordinary positive effect of MID1IP1 abundance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"MID1IP1 siRNA depletion increased AMPK phosphorylation in HepG2 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Hypolipogenic Effect of Shikimic Acid Via Inhibition of MID1IP1 and Phosphorylation of AMPK/ACC. | 2019 | DOI 10.3390/ijms20030582 | PMID 30700011 | https://pubmed.ncbi.nlm.nih.gov/30700011/ | https://doi.org/10.3390/ijms20030582 | https://pmc.ncbi.nlm.nih.gov/articles/PMC6387373/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 81-81; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"4d2d40c5-2491-56a1-a927-a5eba188e8e3","evidence_kind":"source_excerpt","locator":"Lines 81-81","start_line":81,"end_line":81,"excerpt":"**The lipid study contains a useful null result.** Depleting MID1IP1 by siRNA in HepG2 cells reduced SREBP-1c and FAS expression and increased AMPK/ACC phosphorylation. In mouse AML-12 cells, MID1IP1 overexpression attenuated AMPK phosphorylation associated with 80 µM shikimic acid. Conversely, compound C did not alter MID1IP1 expression in the tested HepG2 comparison. This limits a simple claim that AMPK inhibition must reverse every MID1IP1 response. The source tested 10–160 µM SA in viability assays; MTT could fall to about 70% of control at the highest concentration, so reduced lipid staining must not be interpreted without viability context. [Hypolipogenic Effect of Shikimic Acid Via Inhibition of MID1IP1 and Phosphorylation of AMPK/ACC.](https://pubmed.ncbi.nlm.nih.gov/30700011/)","model_system":"Human HepG2 genetic perturbation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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