{"id":"3470072b-8a1b-5511-b128-b6a749b2deb4","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-brain-mito-transport","predicate":"inhibits","statement":"Putrescine and idazoxan inhibited rat brain mitochondrial agmatine uptake despite being ineffective in the compared liver mitochondrial preparation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"4641d445-6b50-507b-a9a0-9059e2e0cc8a","mechanism_event_label":"Compartment and organ identity change which interactions occur.","subject":{"id":"c71bf622-f14b-5314-b1a5-dfa002d2d8ff","slug":"putrescine","display_name":"Putrescine","entity_type_key":"small_molecule"},"object":{"id":"11e0c8ee-bbe0-5fa5-be19-22e54abd977f","slug":"rat-brain-mitochondrial-agmatine-uptake","display_name":"Rat brain mitochondrial agmatine uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4641d445-6b50-507b-a9a0-9059e2e0cc8a","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-brain-mito-transport-event","event_type":"observed_relationship","label":"Compartment and organ identity change which interactions occur.","description":"Putrescine and idazoxan inhibited rat brain mitochondrial agmatine uptake despite being ineffective in the compared liver mitochondrial preparation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c71bf622-f14b-5314-b1a5-dfa002d2d8ff","slug":"putrescine","display_name":"Putrescine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"11e0c8ee-bbe0-5fa5-be19-22e54abd977f","slug":"rat-brain-mitochondrial-agmatine-uptake","display_name":"Rat brain mitochondrial agmatine uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated rat brain versus liver mitochondria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A pharmacological pattern does not establish a particular I2 receptor protein as the transporter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Compartment and organ identity change which interactions occur.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Agmatine transport in brain mitochondria: a different mechanism from that in liver mitochondria. · 2010 · https://pubmed.ncbi.nlm.nih.gov/19997762/ · DOI 10.1007/s00726-009-0401-1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2305e611-9f86-5b89-8609-812b76571759","evidence_kind":"source_excerpt","locator":"Lines 380-386","start_line":380,"end_line":386,"excerpt":"## agmatine-sulfate-brain-mito-transport\nCompartment and organ identity change which interactions occur.\nPutrescine and idazoxan inhibited rat brain mitochondrial agmatine uptake despite being ineffective in the compared liver mitochondrial preparation.\nModel: Isolated rat brain versus liver mitochondria.\nLimitations: A pharmacological pattern does not establish a particular I2 receptor protein as the transporter.\nEvidence access: Primary abstract\nAgmatine transport in brain mitochondria: a different mechanism from that in liver mitochondria. · 2010 · https://pubmed.ncbi.nlm.nih.gov/19997762/ · DOI 10.1007/s00726-009-0401-1","model_system":"Isolated rat brain versus liver mitochondria.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b55d70e22d07b80964f825770d92fdd1745221420ed8af8d94ca5182b9994509","revision_id":"fc7be029-20e0-5943-b74e-3b06ba694571","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}