{"id":"3426b47f-0621-5068-9365-22ef018f602e","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-ado-human-knockdown","predicate":"knockdown_reduces","statement":"RNA-mediated reduction of endogenous human ADO in HepG2/C3A cells decreased hypotaurine production from cysteamine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"deea252f-802c-56d7-b159-3f298c6a7642","mechanism_event_label":"Human-cell gene silencing supports the cysteamine pathway.","subject":{"id":"fdeeed42-76ed-5016-b0bc-c1bf9120af3d","slug":"ado","display_name":"Human 2-aminoethanethiol dioxygenase / ADO","entity_type_key":"protein"},"object":{"id":"eeff5174-9aa8-5f34-b107-e826edde648c","slug":"hypotaurine","display_name":"Hypotaurine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"deea252f-802c-56d7-b159-3f298c6a7642","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-ado-human-knockdown-event","event_type":"observed_relationship","label":"Human-cell gene silencing supports the cysteamine pathway.","description":"RNA-mediated reduction of endogenous human ADO in HepG2/C3A cells decreased hypotaurine production from cysteamine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fdeeed42-76ed-5016-b0bc-c1bf9120af3d","slug":"ado","display_name":"Human 2-aminoethanethiol dioxygenase / ADO","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"eeff5174-9aa8-5f34-b107-e826edde648c","slug":"hypotaurine","display_name":"Hypotaurine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1149e9db-2832-5f4d-aa5b-5aae11bc7788","slug":"cysteamine","display_name":"Cysteamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human liver-derived cultured cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cysteamine can arise through CoA breakdown; this experiment does not show that B5 supplementation raises taurine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Human-cell gene silencing supports the cysteamine pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17581819/ · DOI 10.1074/jbc.M703089200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6b0eba9b-6190-5a16-b001-6193535960d3","evidence_kind":"source_excerpt","locator":"Lines 65-71","start_line":65,"end_line":71,"excerpt":"## taurine-ado-human-knockdown\nHuman-cell gene silencing supports the cysteamine pathway.\nRNA-mediated reduction of endogenous human ADO in HepG2/C3A cells decreased hypotaurine production from cysteamine.\nModel: Human liver-derived cultured cells.\nLimitations: Cysteamine can arise through CoA breakdown; this experiment does not show that B5 supplementation raises taurine.\nEvidence access: Primary abstract\nDiscovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17581819/ · DOI 10.1074/jbc.M703089200","model_system":"Human liver-derived cultured cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c6cb77619a374d9b1b04830032fdd3b33d269d160df5baba3778fad5adf57db5","revision_id":"cc45c491-cc6a-5426-ab72-d7c6d26c3614","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}